RGD Reference Report - The epidermal growth factor receptor tyrosine kinase phosphorylates connexin32. - Rat Genome Database

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The epidermal growth factor receptor tyrosine kinase phosphorylates connexin32.

Authors: Diez, JA  Elvira, M  Villalobo, A 
Citation: Diez JA, etal., Mol Cell Biochem. 1998 Oct;187(1-2):201-10.
RGD ID: 10395247
Pubmed: PMID:9788758   (View Abstract at PubMed)

The epidermal growth factor (EGF) receptor purified by calmodulin-affinity chromatography from solubilized rat liver plasma membranes phosphorylates connexin32 in gap junction plaques isolated from the same origin. Phosphorylation of connexin32 was stimulated by EGF and mainly occurs at tyrosine residue(s), although phosphorylation of serine and threonine residues was also detected. The kinetics parameters for the phosphorylation of connexin32 parallel those for the transphosphorylation of the EGF receptor. m-Calpain proteolyzes phosphoconnexin32, and its major 26 kDa proteolytic fragment only contains phosphotyrosine residue(s). Calmodulin binds to connexin32 in the absence of calcium and prevents in great extent its phosphorylation by the EGF receptor tyrosine kinase.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
peptidyl-tyrosine phosphorylation  IDA 10395247 RGD 
protein autophosphorylation  IDA 10395247 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calmodulin binding  IDA 10395247 RGD 
protein tyrosine kinase activity  IDA 10395247 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Egfr  (epidermal growth factor receptor)


Additional Information