RGD Reference Report - Oxidative stress reveals heterogeneity of FMRP granules in PC12 cell neurites. - Rat Genome Database

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Oxidative stress reveals heterogeneity of FMRP granules in PC12 cell neurites.

Authors: Dolzhanskaya, N  Merz, G  Denman, RB 
Citation: Dolzhanskaya N, etal., Brain Res. 2006 Sep 27;1112(1):56-64. Epub 2006 Aug 17.
RGD ID: 10059582
Pubmed: PMID:16919243   (View Abstract at PubMed)
DOI: DOI:10.1016/j.brainres.2006.07.026   (Journal Full-text)

PC12 cells are a well-known model of parasympathetic neurons. They have also been used to study the dynamics of heterologously expressed fragile X mental retardation (FMRP) granule trafficking down neurites. Here, we demonstrate that undifferentiated and differentiated PC12 cells harbor endogenous FMRP-containing granules. These granules are not stress granules because they do not associate with an authentic stress granule marker protein T-cell internal antigen 1 (TIA-1). Treatment with sodium arsenite induces stress granule formation in undifferentiated and differentiated PC12 cells. In NGF-treated cells, FMRP-containing stress granules are observed in the soma, neurites and growth cones by co-immunostaining with anti-TIA-1 antibody. These data demonstrate that all three microdomains respond similarly to oxidative stress. Nevertheless, we find significantly less co-localization of FMRP and TIA-1 and FMRP and its homologs in the neurites of differentiated PC12 cells treated with sodium arsenite than in the soma or growth cones. The heterogeneity of these granules suggests that FMRP has multiple roles in neurites.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoplasmic stress granule  IDA 10059582 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fmr1  (fragile X messenger ribonucleoprotein 1)


Additional Information