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Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production.

Authors: Kim, TD  Woo, KC  Cho, S  Ha, DC  Jang, SK  Kim, KT 
Citation: Kim TD, etal., Genes Dev. 2007 Apr 1;21(7):797-810.
Pubmed: (View Article at PubMed) PMID:17403780
DOI: Full-text: DOI:10.1101/gad.1519507

The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its species-specific mRNA profiles. Here we show that the circadian timing of AANAT protein expression is regulated by rhythmic translation of AANAT mRNA. This rhythmic control is mediated by both a highly conserved IRES (internal ribosome entry site) element within the AANAT 5' untranslated region and its partner hnRNP Q (heterogeneous nuclear ribonucleoprotein Q) with a peak in the middle of the night. Consistent with the enhancing role of hnRNP Q in AANAT IRES activities, knockdown of the hnRNP Q level elicited a dramatic decrease of peak amplitude in the AANAT protein profile parallel to reduced melatonin production in pinealocytes. This translational regulation of AANAT mRNA provides a novel aspect for achieving the circadian rhythmicity of vertebrate melatonin.


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RGD Object Information
RGD ID: 10059417
Created: 2015-08-17
Species: All species
Last Modified: 2015-08-17
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.