RGD Reference Report - [The changes of inducible nitric oxide synthase activity and apoptosis-related gene expression in endotoxemia-induced rat diaphragm]. - Rat Genome Database

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[The changes of inducible nitric oxide synthase activity and apoptosis-related gene expression in endotoxemia-induced rat diaphragm].

Authors: Fang, YY  Guan, SD  Guo, XL  Ye, HW  Wang, HX  Gao, Q 
Citation: Fang YY, etal., Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2013 May;29(3):209-12.
RGD ID: 10054120
Pubmed: PMID:23940949   (View Abstract at PubMed)

OBJECTIVE: To study the changes of inducible nitric oxide synthase (iNOS) activity and apoptosis-related genes Bcl-2, Bax and caspase-3 mRNA expressions in endotoxemia-induced rat diaphragm injury and analyze the related apoptosis mechanism. METHODS: Thirty-two male SD rats were randomly divided into 4 groups (n = 8): control group (saline 0.5 ml ip), endotoxin 24 h, 48 h and 96 h group (endotoxin 12 mg/kg ip, animals were killed either 24, 48 or 96 h after injections). Body weight were measured, the ratio between diaphragm weight and body weight, activities of constitutive nitric oxide syntheses (cNOS), iNOS and succinate dehydrogenase (SDH) were also measured. The expressions of Bcl-2, Bax and caspase-3 mRNA were detected by RT-PCR analysis. RESULTS: Endotoxin induced significant reductions in diaphragm mass in endotoxin 96 h group (P < 0.05). Endotoxin increased diaphragm cNOS or iNOS activities, and they were significantly higher in endotoxin 96 h group than those in endotoxin 24 h and 48 h groups, diaphragm SDH activity was reduced, and it was lower in endotoxin 96 h group than that in endotoxin 24 h and 48 h groups (P < 0.01). Endotoxin significantly increased Bax and caspase-3 mRNA expressions, and they were higher in endotoxin 48 h and 96 h groups than those in endotoxin 24 h group (P < 0.01). Endotoxin significantly reduced Bcl-2 mRNA expression and the ratio of Bcl-2/Bax, and they were lower in endotoxin 48 h and 96 h groups than those in endotoxin 24 h group (P < 0.01). CONCLUSION: iNOS is activated in endotoxemia-induced rat diaphragm injury. It damages mitochondria, upregulates Bax expression and downregulates Bcl-2 expression, then induces caspase-3 related apoptotic pathway. These changes may cause diaphragm injury and atrophy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Endotoxemia  ISOBax (Rattus norvegicus)10054120; 10054120mRNA:increased expression:diaphragmRGD 
Endotoxemia  ISOBcl2 (Rattus norvegicus)10054120; 10054120mRNA:decreased expression:diaphragmRGD 
Endotoxemia  IEP 10054120; 10054120mRNA:increased expression:diaphragmRGD 
Endotoxemia  IEP 10054120mRNA:decreased expression:diaphragmRGD 
Endotoxemia  ISOCasp3 (Rattus norvegicus)10054120; 10054120mRNA:increased expression:diaphragmRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bax  (BCL2 associated X, apoptosis regulator)
Bcl2  (BCL2, apoptosis regulator)
Casp3  (caspase 3)

Genes (Mus musculus)
Bax  (BCL2-associated X protein)
Bcl2  (B cell leukemia/lymphoma 2)
Casp3  (caspase 3)

Genes (Homo sapiens)
BAX  (BCL2 associated X, apoptosis regulator)
BCL2  (BCL2 apoptosis regulator)
CASP3  (caspase 3)


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