RGD Reference Report - Ferulic acid prevents the cerebral ischemic injury-induced decrease of Akt and Bad phosphorylation. - Rat Genome Database
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Ferulic acid prevents the cerebral ischemic injury-induced decrease of Akt and Bad phosphorylation.

Authors: Koh, PO 
Citation: Koh PO Neurosci Lett. 2012 Jan 24;507(2):156-60. doi: 10.1016/j.neulet.2011.12.012. Epub 2011 Dec 17.
RGD ID: 10053665
Pubmed: (View Article at PubMed) PMID:22200499
DOI: Full-text: DOI:10.1016/j.neulet.2011.12.012

Ferulic acid protects neuronal cells from glutamate-induced excitotoxicity and focal cerebral ischemia. This study investigated whether ferulic acid exerts a neuroprotective effect through the activation of Akt and its downstream targets, Bad and 14-3-3. Adult male rats were immediately treated with ferulic acid (100mg/kg, i.v.) after middle cerebral artery occlusion (MCAO). Brains were collected 24h after MCAO and infarct volumes were analyzed using triphenyltetrazolium chloride staining. It was found that ferulic acid treatment significantly reduced infarct volume during MCAO. Ferulic acid attenuated the MCAO injury-induced decrease of phospho-PDK1, phospho-Akt and phospho-Bad levels. However, ferulic acid did not affect the expression of 14-3-3 and Bcl-xL, which exerts an anti-apoptotic effect through interaction with phospho-Bad. Immunoprecipitation analysis demonstrated that the interaction between phospho-Bad and 14-3-3 decreased during MCAO, whereas ferulic acid prevented the injury-induced decrease in these interaction levels. Moreover, ferulic acid prevented the injury-induced increase in cleaved caspase-3 levels. These findings suggest that ferulic acid attenuates cell death during MCAO and that these protective effects are due to inhibition of Akt signaling pathway inactivation and maintenance of the interaction between phospho-Bad and 14-3-3.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Bad  (BCL2-associated agonist of cell death)

Genes (Mus musculus)
Bad  (BCL2-associated agonist of cell death)

Genes (Homo sapiens)
BAD  (BCL2 associated agonist of cell death)


Additional Information