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Inhibitory effect of baicalin on iNOS and NO expression in intestinal mucosa of rats with acute endotoxemia.

Authors: Feng, A  Zhou, G  Yuan, X  Huang, X  Zhang, Z  Zhang, T 
Citation: Feng A, etal., PLoS One. 2013 Dec 2;8(12):e80997. doi: 10.1371/journal.pone.0080997. eCollection 2013.
Pubmed: (View Article at PubMed) PMID:24312512
DOI: Full-text: DOI:10.1371/journal.pone.0080997

The mechanism by which baicalin modulated the expression of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in the mucosa of distal ileum was investigated in a rat model of acute endo-toxemia induced by intraperitoneal injection of bacterial lipopolysaccharide (LPS). The experiment demonstrated that LPS upregulated iNOS mRNA and protein expression as well as NO produc-tion (measured as the stable degradation production, nitrites). LPS not only increased toll-like receptor 4 (TLR4) and peroxisome proliferator-activated receptor gamma (PPARgamma) content, but also activated p38 and activating transcription factor 2 (ATF2) and inactivated PPARgamma via phosphorylation. Inhibition of p38 signalling pathway by chemical inhibitor SB202190 and small interfering RNA (siRNA) ameliorated LPS-induced iNOS generation, while suppression of PPARgamma pathway by SR-202 boosted LPS-elicited iNOS expression. Baicalin treatment (I) attenuated LPS-induced iNOS mRNA and protein as well as nitrites generation, and (II) ameliorated LPS-elicited TLR4 and PPARgamma production, and (III) inhibited p38/ATF2 phosphorylation leading to suppression of p38 signalling, and (IV) prevented PPARgamma from phosphorylation contributing to maintainence of PPARgamma bioactivity. However, SR-202 co-treatment (I) partially abrogated the inhibitory effect of baicalin on iNOS mRNA expression, and (II) partially reversed baicalin-inhibited p38 phosphorylation. In summary, baicalin could ameliorate LPS-induced iNOS and NO overproduction in mucosa of rat terminal ileum via inhibition of p38 signalling cascade and activation of PPARgamma pathway. There existed a interplay between the two signalling pathways.

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RGD Object Information
RGD ID: 10047418
Created: 2015-07-13
Species: All species
Last Modified: 2015-07-13
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.