RGD Reference Report - Oligomerization properties of ERp29, an endoplasmic reticulum stress protein. - Rat Genome Database

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Oligomerization properties of ERp29, an endoplasmic reticulum stress protein.

Authors: Mkrtchian, S  Baryshev, M  Matvijenko, O  Sharipo, A  Sandalova, T  Schneider, G  Ingelman-Sundberg, M 
Citation: Mkrtchian S, etal., FEBS Lett. 1998 Jul 24;431(3):322-6.
RGD ID: 10047223
Pubmed: PMID:9714535   (View Abstract at PubMed)

ERp29, a novel and ubiquitously expressed endoplasmic reticulum (ER) stress-inducible protein, was recently isolated and cDNA cloned in our laboratory. Using size exclusion chromatography and chemical cross-linking we have assessed the oligomerization properties of ERp29. Purified ERp29 in solution as well as in rat hepatoma cells self-associates predominantly into homodimers. Labeling of the cells with [35S]methionine with subsequent cross-linking and immunoprecipitation showed that ERp29 interacts with a number of ER proteins, one of which was previously identified as BiP/GRP78. Secondary structure prediction and fold recognition methods indicate that the native conformation of ERp29 resembles the thioredoxin fold, a structural motif characteristic of a number of enzymes with the redox function, including protein disulfide isomerase (with which ERp29 shares limited sequence similarity). Dimerization of the protein is suggested to be advantageous for the protein binding potential of ERp29.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to endoplasmic reticulum stress involved_inNAS 10047223PMID:9714535ParkinsonsUK-UCL 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P5255510047223PMID:9714535ParkinsonsUK-UCL 
protein homodimerization activity enablesIPIUniProtKB:P5255510047223PMID:9714535ParkinsonsUK-UCL 
protein-folding chaperone binding enablesIPIUniProtKB:P0676110047223PMID:9714535ParkinsonsUK-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Erp29  (endoplasmic reticulum protein 29)
Hspa5  (heat shock protein family A (Hsp70) member 5)


Additional Information