RGD Reference Report - Anoctamin 1 is apically expressed on thyroid follicular cells and contributes to ATP- and calcium-activated iodide efflux. - Rat Genome Database

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Anoctamin 1 is apically expressed on thyroid follicular cells and contributes to ATP- and calcium-activated iodide efflux.

Authors: Iosco, C  Cosentino, C  Sirna, L  Romano, R  Cursano, S  Mongia, A  Pompeo, G  Di Bernardo, J  Ceccarelli, C  Tallini, G  Rhoden, KJ 
Citation: Iosco C, etal., Cell Physiol Biochem. 2014;34(3):966-80. doi: 10.1159/000366313. Epub 2014 Aug 27.
RGD ID: 10047211
Pubmed: PMID:25201006   (View Abstract at PubMed)
DOI: DOI:10.1159/000366313   (Journal Full-text)

BACKGROUND/AIMS: Iodide efflux from thyroid cells into the follicular lumen is essential for the synthesis of thyroid hormones, however, the pathways mediating this transport have only been partially identified. A calcium-activated pathway of iodide efflux has long been recognized, but its molecular identity unknown. Anoctamin 1 (ANO1) is a calcium-activated chloride channel (CaCC), and this study aims to investigate its contribution to iodide fluxes in thyroid cells. METHODS: RT-PCR, immunohistochemistry, and live cell imaging with the fluorescent halide biosensor YFP-H148Q/I152L were used to study the expression, localization and function of ANO1 in thyroid cells. RESULTS: ANO1 mRNA was detected in human thyroid tissue and FRTL-5 thyrocytes, and ANO1 protein was localized to the apical membrane of follicular cells. ATP induced a transient loss of iodide from FRTL-5 cells that was dependent on the mobilization of intracellular calcium, and was inhibited by CaCC/ANO1 inhibitors and siRNA against ANO1. Calcium-activated iodide efflux was also observed in CHO cells over-expressing the Sodium Iodide Symporter (NIS) and ANO1. CONCLUSION: ANO1 in thyrocytes functions as a calcium-activated channel mediating iodide efflux, and may contribute to the rapid delivery of iodide into the follicular lumen for the synthesis of thyroid hormones following activation by calcium-mobilizing stimuli.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ano1Ratchloride transport involved_inIMP PMID:25201006UniProt 
Ano1Ratiodide transport involved_inIMP PMID:25201006UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ano1Ratintracellularly calcium-gated chloride channel activity enablesIMP PMID:25201006UniProt 
Ano1Ratiodide transmembrane transporter activity enablesIMP PMID:25201006UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Ano1  (anoctamin 1)


Additional Information