RGD Reference Report - PGE2 inhibits basolateral 50 pS potassium channels in the thick ascending limb of the rat kidney. - Rat Genome Database

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PGE2 inhibits basolateral 50 pS potassium channels in the thick ascending limb of the rat kidney.

Authors: Gu, R  Jin, Y  Zhai, Y  Yang, L  Zhang, C  Li, W  Wang, L  Kong, S  Zhang, Y  Yang, B  Wang, WH 
Citation: Gu R, etal., Kidney Int. 2008 Aug;74(4):478-85. doi: 10.1038/ki.2008.198. Epub 2008 May 21.
RGD ID: 10043331
Pubmed: (View Article at PubMed) PMID:18496512
DOI: Full-text: DOI:10.1038/ki.2008.198

To study the inhibition of the inwardly rectifying basolateral 50 pS potassium channels by PGE(2) we performed patch-clamp studies on the basolateral membrane of the rat kidney thick ascending limb. PGE(2)'s effect was mimicked by the selective EP1- and EP3-receptor agonist, sulprostone, but was prevented by inhibiting protein kinase-C with calphostin-C. The mitogen-activated protein kinase inhibitor PD98059 (ERK) or SB203580 (p38) increased basal channel activity; however, while neither alone prevented the inhibitory effect of PGE(2), but using both of them together completely abolished PGE(2)'s effect on channel activity. Treatment with PGE(2) stimulated phosphorylation of both p38 and ERK in primary cultures of medullary thick ascending limb cells. The PGE(2)-mediated mitogen-activated protein kinase activation was not affected by indomethacin, but was completely blocked by calphostin-C. These studies show that inhibition of basolateral 50 pS potassium channels by PGE(2) is mediated by protein kinase-C, which in turn stimulates mitogen-activated protein kinases in the thick ascending limb of the rat kidney.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Ptger3  (prostaglandin E receptor 3)


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