RGD Reference Report - Effects of herpes simplex virus vector-mediated enkephalin gene therapy on bladder overactivity and nociception. - Rat Genome Database

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Effects of herpes simplex virus vector-mediated enkephalin gene therapy on bladder overactivity and nociception.

Authors: Yokoyama, H  Oguchi, T  Goins, WF  Goss, JR  Nishizawa, O  De Groat, WC  Wolfe, D  Krisky, DM  Glorioso, JC  Yoshimura, N 
Citation: Yokoyama H, etal., Hum Gene Ther. 2013 Feb;24(2):170-80. doi: 10.1089/hum.2011.180. Epub 2013 Feb 14.
RGD ID: 10003040
Pubmed: PMID:23316929   (View Abstract at PubMed)
PMCID: PMC3581021   (View Article at PubMed Central)
DOI: DOI:10.1089/hum.2011.180   (Journal Full-text)

We previously reported the effects of herpes simplex virus (HSV) vector-mediated enkephalin on bladder overactivity and pain. In this study, we evaluated the effects of vHPPE (E1G6-ENK), a newly engineered replication-deficient HSV vector encoding human preproenkephalin (hPPE). vHPPE or control vector was injected into the bladder wall of female rats 2 weeks prior to the following studies. A reverse-transcription PCR study showed high hPPE transgene levels in L6 dorsal root ganglia innervating the bladder in the vHPPE group. The number of freezing behaviors, which is a nociceptive reaction associated with bladder pain, was also significantly lower in the vHPPE group compared with the control group. The number of L6 spinal cord c-fos-positive cells and the urinary interleukin (IL)-1beta and IL-6 levels after resiniferatoxin (RTx) administration into the bladder of the vHPPE group were significantly lower compared with those of the control vector-injected group. In continuous cystometry, the vHPPE group showed a smaller reduction in intercontraction interval after RTx administration into the bladder. This antinociceptive effect was antagonized by naloxone hydrochloride. Thus, the HSV vector vHPPE encoding hPPE demonstrated physiological improvement in visceral pain induced by bladder irritation. Gene therapy may represent a potentially useful treatment modality for bladder hypersensitive disorders such as bladder pain syndrome/interstitial cystitis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
overactive bladder syndrome treatmentIDA 10003040 RGD 
overactive bladder syndrome treatmentISOPENK (Homo sapiens)10003040; 10003040 RGD 
Visceral Pain treatmentIDA 10003040 RGD 
Visceral Pain treatmentISOPENK (Homo sapiens)10003040; 10003040 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Penk  (proenkephalin)

Genes (Mus musculus)
Penk  (preproenkephalin)

Genes (Homo sapiens)
PENK  (proenkephalin)


Additional Information