RGD Reference Report - Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits. - Rat Genome Database

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Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits.

Authors: O'Donohue, MF  Choesmel, V  Faubladier, M  Fichant, G  Gleizes, PE 
Citation: O'Donohue MF, etal., J Cell Biol. 2010 Sep 6;190(5):853-66. doi: 10.1083/jcb.201005117.
RGD ID: 10002730
Pubmed: PMID:20819938   (View Abstract at PubMed)
PMCID: PMC2935573   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.201005117   (Journal Full-text)

Our knowledge of the functions of metazoan ribosomal proteins in ribosome synthesis remains fragmentary. Using siRNAs, we show that knockdown of 31 of the 32 ribosomal proteins of the human 40S subunit (ribosomal protein of the small subunit [RPS]) strongly affects pre-ribosomal RNA (rRNA) processing, which often correlates with nucleolar chromatin disorganization. 16 RPSs are strictly required for initiating processing of the sequences flanking the 18S rRNA in the pre-rRNA except at the metazoan-specific early cleavage site. The remaining 16 proteins are necessary for progression of the nuclear and cytoplasmic maturation steps and for nuclear export. Distribution of these two subsets of RPSs in the 40S subunit structure argues for a tight dependence of pre-rRNA processing initiation on the folding of both the body and the head of the forming subunit. Interestingly, the functional dichotomy of RPS proteins reported in this study is correlated with the mutation frequency of RPS genes in Diamond-Blackfan anemia.



Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FAUHumanribosome biogenesis pathway   IMP  RGD 
FauRatribosome biogenesis pathway   ISOFAU (Homo sapiens) RGD 
FauMouseribosome biogenesis pathway   ISOFAU (Homo sapiens) RGD 
RPS10Humanribosome biogenesis pathway   IMP  RGD 
RPS11Humanribosome biogenesis pathway   IMP  RGD 
RPS12Humanribosome biogenesis pathway   IMP  RGD 
RPS13Humanribosome biogenesis pathway   IMP  RGD 
RPS14Humanribosome biogenesis pathway   IMP  RGD 
RPS15Humanribosome biogenesis pathway   IMP  RGD 
RPS15AHumanribosome biogenesis pathway   IMP  RGD 
RPS16Humanribosome biogenesis pathway   IMP  RGD 
RPS17Humanribosome biogenesis pathway   IMP  RGD 
RPS18Humanribosome biogenesis pathway   IMP  RGD 
RPS19Humanribosome biogenesis pathway   IMP  RGD 
RPS2Humanribosome biogenesis pathway   IMP  RGD 
RPS20Humanribosome biogenesis pathway   IMP  RGD 
RPS21Humanribosome biogenesis pathway   IMP  RGD 
RPS23Humanribosome biogenesis pathway   IMP  RGD 
RPS24Humanribosome biogenesis pathway   IMP  RGD 
RPS25Humanribosome biogenesis pathway   IMP  RGD 
RPS26Humanribosome biogenesis pathway   IMP  RGD 
RPS27Humanribosome biogenesis pathway   IMP  RGD 
RPS27AHumanribosome biogenesis pathway   IMP  RGD 
RPS27LHumanribosome biogenesis pathway   IMP  RGD 
RPS28Humanribosome biogenesis pathway   IMP  RGD 
RPS29Humanribosome biogenesis pathway   IMP  RGD 
RPS3Humanribosome biogenesis pathway   IMP  RGD 
RPS3AHumanribosome biogenesis pathway   IMP  RGD 
RPS4XHumanribosome biogenesis pathway   IMP  RGD 
RPS4Y1Humanribosome biogenesis pathway   TAS  RGD 
RPS4Y2Humanribosome biogenesis pathway   TAS  RGD 
RPS5Humanribosome biogenesis pathway   IMP  RGD 
RPS6Humanribosome biogenesis pathway   IMP  RGD 
RPS7Humanribosome biogenesis pathway   IMP  RGD 
RPS8Humanribosome biogenesis pathway   IMP  RGD 
RPS9Humanribosome biogenesis pathway   IMP  RGD 
RPSAHumanribosome biogenesis pathway   IMP  RGD 
Rps10Ratribosome biogenesis pathway   ISORPS10 (Homo sapiens) RGD 
Rps10Mouseribosome biogenesis pathway   ISORPS10 (Homo sapiens) RGD 
Rps11Ratribosome biogenesis pathway   ISORPS11 (Homo sapiens) RGD 
Rps11Mouseribosome biogenesis pathway   ISORPS11 (Homo sapiens) RGD 
Rps12Ratribosome biogenesis pathway   ISORPS12 (Homo sapiens) RGD 
Rps12Mouseribosome biogenesis pathway   ISORPS12 (Homo sapiens) RGD 
Rps13Ratribosome biogenesis pathway   ISORPS13 (Homo sapiens) RGD 
Rps13Mouseribosome biogenesis pathway   ISORPS13 (Homo sapiens) RGD 
Rps14Ratribosome biogenesis pathway   ISORPS14 (Homo sapiens) RGD 
Rps14Mouseribosome biogenesis pathway   ISORPS14 (Homo sapiens) RGD 
Rps15Ratribosome biogenesis pathway   ISORPS15 (Homo sapiens) RGD 
Rps15Mouseribosome biogenesis pathway   ISORPS15 (Homo sapiens) RGD 
Rps15aRatribosome biogenesis pathway   ISORPS15A (Homo sapiens) RGD 
Rps15aMouseribosome biogenesis pathway   ISORPS15A (Homo sapiens) RGD 
Rps16Ratribosome biogenesis pathway   ISORPS16 (Homo sapiens) RGD 
Rps16Mouseribosome biogenesis pathway   ISORPS16 (Homo sapiens) RGD 
Rps17Mouseribosome biogenesis pathway   ISORPS17 (Homo sapiens) RGD 
Rps18Ratribosome biogenesis pathway   ISORPS18 (Homo sapiens) RGD 
Rps18Mouseribosome biogenesis pathway   ISORPS18 (Homo sapiens) RGD 
Rps19Ratribosome biogenesis pathway   ISORPS19 (Homo sapiens) RGD 
Rps2Ratribosome biogenesis pathway   ISORPS2 (Homo sapiens) RGD 
Rps2Mouseribosome biogenesis pathway   ISORPS2 (Homo sapiens) RGD 
Rps20Ratribosome biogenesis pathway   ISORPS20 (Homo sapiens) RGD 
Rps20Mouseribosome biogenesis pathway   ISORPS20 (Homo sapiens) RGD 
Rps21Ratribosome biogenesis pathway   ISORPS21 (Homo sapiens) RGD 
Rps21Mouseribosome biogenesis pathway   ISORPS21 (Homo sapiens) RGD 
Rps23Ratribosome biogenesis pathway   ISORPS23 (Homo sapiens) RGD 
Rps23Mouseribosome biogenesis pathway   ISORPS23 (Homo sapiens) RGD 
Rps24Ratribosome biogenesis pathway   ISORPS24 (Homo sapiens) RGD 
Rps24Mouseribosome biogenesis pathway   ISORPS24 (Homo sapiens) RGD 
Rps25Ratribosome biogenesis pathway   ISORPS25 (Homo sapiens) RGD 
Rps25Mouseribosome biogenesis pathway   ISORPS25 (Homo sapiens) RGD 
Rps26Ratribosome biogenesis pathway   ISORPS26 (Homo sapiens) RGD 
Rps27Ratribosome biogenesis pathway   ISORPS27 (Homo sapiens) RGD 
Rps27aMouseribosome biogenesis pathway   ISORPS27A (Homo sapiens) RGD 
Rps27aRatribosome biogenesis pathway   ISORPS27A (Homo sapiens) RGD 
Rps27lRatribosome biogenesis pathway   ISORPS27L (Homo sapiens) RGD 
Rps27lMouseribosome biogenesis pathway   ISORPS27L (Homo sapiens) RGD 
Rps28Ratribosome biogenesis pathway   ISORPS28 (Homo sapiens) RGD 
Rps28Mouseribosome biogenesis pathway   ISORPS28 (Homo sapiens) RGD 
Rps29Ratribosome biogenesis pathway   ISORPS29 (Homo sapiens) RGD 
Rps29Mouseribosome biogenesis pathway   ISORPS29 (Homo sapiens) RGD 
Rps3Ratribosome biogenesis pathway   ISORPS3 (Homo sapiens) RGD 
Rps3Mouseribosome biogenesis pathway   ISORPS3 (Homo sapiens) RGD 
Rps3aRatribosome biogenesis pathway   ISORPS3A (Homo sapiens) RGD 
Rps3a1Mouseribosome biogenesis pathway   ISORPS3A (Homo sapiens) RGD 
Rps4xMouseribosome biogenesis pathway   ISORPS4X (Homo sapiens) RGD 
Rps4xRatribosome biogenesis pathway   ISORPS4X (Homo sapiens) RGD 
Rps4y2Ratribosome biogenesis pathway   ISORPS4Y2 (Homo sapiens) RGD 
Rps5Ratribosome biogenesis pathway   ISORPS5 (Homo sapiens) RGD 
Rps5Mouseribosome biogenesis pathway   ISORPS5 (Homo sapiens) RGD 
Rps6Mouseribosome biogenesis pathway   ISORPS6 (Homo sapiens) RGD 
Rps7Ratribosome biogenesis pathway   ISORPS7 (Homo sapiens) RGD 
Rps7Mouseribosome biogenesis pathway   ISORPS7 (Homo sapiens) RGD 
Rps8Ratribosome biogenesis pathway   ISORPS8 (Homo sapiens) RGD 
Rps8Mouseribosome biogenesis pathway   ISORPS8 (Homo sapiens) RGD 
Rps9Mouseribosome biogenesis pathway   ISORPS9 (Homo sapiens) RGD 
RpsaRatribosome biogenesis pathway   ISORPSA (Homo sapiens) RGD 
RpsaMouseribosome biogenesis pathway   ISORPSA (Homo sapiens) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Fau  (FAU ubiquitin like and ribosomal protein S30 fusion)
Rps10  (ribosomal protein S10)
Rps11  (ribosomal protein S11)
Rps12  (ribosomal protein S12)
Rps13  (ribosomal protein S13)
Rps14  (ribosomal protein S14)
Rps15  (ribosomal protein S15)
Rps15a  (ribosomal protein S15a)
Rps16  (ribosomal protein S16)
Rps18  (ribosomal protein S18)
Rps19  (ribosomal protein S19)
Rps2  (ribosomal protein S2)
Rps20  (ribosomal protein S20)
Rps21  (ribosomal protein S21)
Rps23  (ribosomal protein S23)
Rps24  (ribosomal protein S24)
Rps25  (ribosomal protein s25)
Rps26  (ribosomal protein S26)
Rps27  (ribosomal protein S27)
Rps27a  (ribosomal protein S27a)
Rps27l  (ribosomal protein S27-like)
Rps28  (ribosomal protein S28)
Rps29  (ribosomal protein S29)
Rps3  (ribosomal protein S3)
Rps3a  (ribosomal protein S3a)
Rps4x  (ribosomal protein S4, X-linked)
Rps4y2  (ribosomal protein S4, Y-linked 2)
Rps5  (ribosomal protein S5)
Rps7  (ribosomal protein S7)
Rps8  (ribosomal protein S8)
Rpsa  (ribosomal protein SA)

Genes (Mus musculus)
Fau  (FAU ubiquitin like and ribosomal protein S30 fusion)
Rps10  (ribosomal protein S10)
Rps11  (ribosomal protein S11)
Rps12  (ribosomal protein S12)
Rps13  (ribosomal protein S13)
Rps14  (ribosomal protein S14)
Rps15  (ribosomal protein S15)
Rps15a  (ribosomal protein S15A)
Rps16  (ribosomal protein S16)
Rps17  (ribosomal protein S17)
Rps18  (ribosomal protein S18)
Rps2  (ribosomal protein S2)
Rps20  (ribosomal protein S20)
Rps21  (ribosomal protein S21)
Rps23  (ribosomal protein S23)
Rps24  (ribosomal protein S24)
Rps25  (ribosomal protein S25)
Rps27a  (ribosomal protein S27A)
Rps27l  (ribosomal protein S27-like)
Rps28  (ribosomal protein S28)
Rps29  (ribosomal protein S29)
Rps3  (ribosomal protein S3)
Rps3a1  (ribosomal protein S3A1)
Rps4x  (ribosomal protein S4, X-linked)
Rps5  (ribosomal protein S5)
Rps6  (ribosomal protein S6)
Rps7  (ribosomal protein S7)
Rps8  (ribosomal protein S8)
Rps9  (ribosomal protein S9)
Rpsa  (ribosomal protein SA)

Genes (Homo sapiens)
FAU  (FAU ubiquitin like and ribosomal protein S30 fusion)
RPS10  (ribosomal protein S10)
RPS11  (ribosomal protein S11)
RPS12  (ribosomal protein S12)
RPS13  (ribosomal protein S13)
RPS14  (ribosomal protein S14)
RPS15  (ribosomal protein S15)
RPS15A  (ribosomal protein S15a)
RPS16  (ribosomal protein S16)
RPS17  (ribosomal protein S17)
RPS18  (ribosomal protein S18)
RPS19  (ribosomal protein S19)
RPS2  (ribosomal protein S2)
RPS20  (ribosomal protein S20)
RPS21  (ribosomal protein S21)
RPS23  (ribosomal protein S23)
RPS24  (ribosomal protein S24)
RPS25  (ribosomal protein S25)
RPS26  (ribosomal protein S26)
RPS27  (ribosomal protein S27)
RPS27A  (ribosomal protein S27a)
RPS27L  (ribosomal protein S27 like)
RPS28  (ribosomal protein S28)
RPS29  (ribosomal protein S29)
RPS3  (ribosomal protein S3)
RPS3A  (ribosomal protein S3A)
RPS4X  (ribosomal protein S4 X-linked)
RPS4Y1  (ribosomal protein S4 Y-linked 1)
RPS4Y2  (ribosomal protein S4 Y-linked 2)
RPS5  (ribosomal protein S5)
RPS6  (ribosomal protein S6)
RPS7  (ribosomal protein S7)
RPS8  (ribosomal protein S8)
RPS9  (ribosomal protein S9)
RPSA  (ribosomal protein SA)


Additional Information