Mir222 (microRNA 222) - Rat Genome Database

Send us a Message

Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Gene: Mir222 (microRNA 222) Mus musculus
Symbol: Mir222
Name: microRNA 222
RGD ID: 1608223
Description: Acts upstream of or within several processes, including cellular response to forskolin; cellular response to tumor necrosis factor; and long-term synaptic potentiation. Part of RISC complex. Is expressed in lung. Human ortholog(s) of this gene implicated in colorectal cancer; gastrointestinal stromal tumor; lung cancer; and oral squamous cell carcinoma. Orthologous to human MIR222 (microRNA 222).
Type: ncrna (Ensembl: miRNA)
Previously known as: mir-222; Mirn; Mirn222; mmu-mir-2; mmu-mir-222
RGD Orthologs
Alliance Genes
More Info more info ...
Latest Assembly: GRCm39 - Mouse Genome Assembly GRCm39
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
GRCm39X19,013,132 - 19,013,210 (-)NCBIGRCm39GRCm39mm39
GRCm39 EnsemblX19,013,132 - 19,013,210 (-)EnsemblGRCm39 Ensembl
GRCm38X19,146,893 - 19,146,971 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 EnsemblX19,146,893 - 19,146,971 (-)EnsemblGRCm38mm10GRCm38
MGSCv37X18,724,019 - 18,724,097 (-)NCBIGRCm37MGSCv37mm9NCBIm37
CeleraX16,723,051 - 16,723,129 (-)NCBICelera
Cytogenetic MapXA1.3NCBI
JBrowse: View Region in Genome Browser (JBrowse)

Gene Ontology Annotations     Click to see Annotation Detail View

Biological Process
angiogenesis involved in wound healing  (ISO)
cellular response to amino acid stimulus  (IDA)
cellular response to decreased oxygen levels  (IEP)
cellular response to forskolin  (IEP)
cellular response to glucose stimulus  (ISO)
cellular response to hypoxia  (ISO)
cellular response to inorganic substance  (IDA)
cellular response to nerve growth factor stimulus  (ISO)
cellular response to polyamine macromolecule  (ISO)
cellular response to tumor necrosis factor  (IDA)
lactation  (ISO)
long-term synaptic potentiation  (IEP)
mesenchymal cell differentiation involved in bone development  (ISO)
miRNA-mediated gene silencing  (ISO)
miRNA-mediated gene silencing by inhibition of translation  (ISO)
negative regulation of angiogenesis  (ISO)
negative regulation of apoptotic process  (ISO)
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis  (ISO)
negative regulation of cardiac muscle cell apoptotic process  (ISO)
negative regulation of cell adhesion molecule production  (ISO)
negative regulation of epithelial cell migration  (ISO)
negative regulation of epithelial cell proliferation  (ISO)
negative regulation of gene expression  (ISO)
negative regulation of hematopoietic stem cell proliferation  (ISO)
negative regulation of inflammatory response  (ISO)
negative regulation of interleukin-21 production  (ISO)
negative regulation of leukocyte adhesion to vascular endothelial cell  (ISO)
negative regulation of neuron apoptotic process  (ISO)
negative regulation of ossification  (ISO)
negative regulation of osteoblast differentiation  (ISO)
negative regulation of progesterone secretion  (ISO)
negative regulation of TRAIL-activated apoptotic signaling pathway  (ISO)
negative regulation of wound healing  (ISO)
positive regulation of autophagy  (ISO)
positive regulation of cardiac muscle cell proliferation  (ISO)
positive regulation of cell size  (ISO)
positive regulation of dendrite extension  (ISO)
positive regulation of epithelial cell apoptotic process  (ISO)
positive regulation of epithelial cell migration  (ISO)
positive regulation of epithelial to mesenchymal transition  (ISO)
positive regulation of ERK1 and ERK2 cascade  (ISO)
positive regulation of erythrocyte differentiation  (ISO)
positive regulation of estradiol secretion  (ISO)
positive regulation of follicle-stimulating hormone secretion  (ISO)
positive regulation of G1/S transition of mitotic cell cycle  (ISO)
positive regulation of luteinizing hormone secretion  (ISO)
positive regulation of prolactin secretion  (ISO)
positive regulation of protein kinase B signaling  (ISO)
positive regulation of Schwann cell migration  (ISO)
positive regulation of Schwann cell proliferation  (ISO)
positive regulation of testosterone secretion  (ISO)
positive regulation of vascular associated smooth muscle cell proliferation  (ISO)
response to ischemia  (ISO)
response to nutrient levels  (ISO)
response to oxygen levels  (IDA)
response to xenobiotic stimulus  (ISO)
sensory perception of sound  (IEP)
Wnt signaling pathway  (IDA)

Cellular Component

Molecular Function

Molecular Pathway Annotations     Click to see Annotation Detail View

References - curated
# Reference Title Reference Citation
1. Acupuncture protects the interstitial cells of Cajal by regulating miR-222 in a rat model of post-operative ileus. Deng JJ, etal., Acupunct Med. 2019 Apr;37(2):125-132. doi: 10.1177/0964528419829755. Epub 2019 Mar 11.
2. Evaluation of circulating miR-21 and miR-222 as diagnostic biomarkers for gastric cancer. Emami SS, etal., J Cancer Res Ther. 2019 Jan-Mar;15(1):115-119. doi: 10.4103/jcrt.JCRT_592_17.
3. miR-221-3p and miR-222-3p downregulation promoted osteogenic differentiation of bone marrow mesenchyme stem cells through IGF-1/ERK pathway under high glucose condition. Gan K, etal., Diabetes Res Clin Pract. 2020 Sep;167:108121. doi: 10.1016/j.diabres.2020.108121. Epub 2020 Mar 16.
4. Inhibition of microRNA-222 up-regulates TIMP3 to promotes osteogenic differentiation of MSCs from fracture rats with type 2 diabetes mellitus. Jiang C, etal., J Cell Mol Med. 2020 Jan;24(1):686-694. doi: 10.1111/jcmm.14777. Epub 2019 Nov 6.
5. Correlation of the Levels of microRNA with Cell Composition of Axillary Lymph Node in Wistar Rats with Breast Cancer. Kabakov AV, etal., Bull Exp Biol Med. 2020 Feb;168(4):500-502. doi: 10.1007/s10517-020-04740-w. Epub 2020 Mar 10.
6. Down-regulation of miR-221 and miR-222 correlates with pronounced Kit expression in gastrointestinal stromal tumors. Koelz M, etal., Int J Oncol. 2011 Feb;38(2):503-11. doi: 10.3892/ijo.2010.857. Epub 2010 Dec 3.
7. Altered microRNA regulation in Huntington's disease models. Lee ST, etal., Exp Neurol. 2011 Jan;227(1):172-9. doi: 10.1016/j.expneurol.2010.10.012. Epub 2010 Oct 28.
8. Negative correlation of cytoplasm TIMP3 with miR-222 indicates a good prognosis for NSCLC. Lei Y, etal., Onco Targets Ther. 2018 Sep 6;11:5551-5557. doi: 10.2147/OTT.S172522. eCollection 2018.
9. lncRNA GAS5 Inhibits Cell Migration and Invasion and Promotes Autophagy by Targeting miR-222-3p via the GAS5/PTEN-Signaling Pathway in CRC. Liu L, etal., Mol Ther Nucleic Acids. 2019 Sep 6;17:644-656. doi: 10.1016/j.omtn.2019.06.009. Epub 2019 Jun 27.
10. A microRNA 221- and 222-mediated feedback loop maintains constitutive activation of NFκB and STAT3 in colorectal cancer cells. Liu S, etal., Gastroenterology. 2014 Oct;147(4):847-859.e11. doi: 10.1053/j.gastro.2014.06.006. Epub 2014 Jun 12.
11. miR-222 is necessary for exercise-induced cardiac growth and protects against pathological cardiac remodeling. Liu X, etal., Cell Metab. 2015 Apr 7;21(4):584-95. doi: 10.1016/j.cmet.2015.02.014.
12. MicroRNA-222 expression and its prognostic potential in non-small cell lung cancer. Mao KP, etal., ScientificWorldJournal. 2014;2014:908326. doi: 10.1155/2014/908326. Epub 2014 Apr 13.
13. Upregulation of miR-222 in both Helicobacter pylori- infected and noninfected gastric cancer patients. Noormohammad M, etal., J Genet. 2016 Dec;95(4):991-995. doi: 10.1007/s12041-016-0728-9.
14. Serum microRNA profiles as prognostic/predictive markers in the multimodality therapy of locally advanced adenocarcinomas of the gastroesophageal junction. Odenthal M, etal., Int J Cancer. 2015 Jul 1;137(1):230-7. doi: 10.1002/ijc.29363. Epub 2014 Dec 3.
15. MicroRNA-221/222 upregulation indicates the activation of stellate cells and the progression of liver fibrosis. Ogawa T, etal., Gut. 2012 Nov;61(11):1600-9. doi: 10.1136/gutjnl-2011-300717. Epub 2012 Jan 20.
16. MicroRNA deregulation in human thyroid papillary carcinomas. Pallante P, etal., Endocr Relat Cancer. 2006 Jun;13(2):497-508. doi: 10.1677/erc.1.01209.
17. The rs17084733 variant in the KIT 3' UTR disrupts a miR-221/222 binding site in gastrointestinal stromal tumour: a sponge-like mechanism conferring disease susceptibility. Ravegnini G, etal., Epigenetics. 2019 Jun;14(6):545-557. doi: 10.1080/15592294.2019.1595997. Epub 2019 Apr 13.
18. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
19. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
20. Cardiac-Specific Overexpression of miR-222 Induces Heart Failure and Inhibits Autophagy in Mice. Su M, etal., Cell Physiol Biochem. 2016;39(4):1503-11. doi: 10.1159/000447853. Epub 2016 Sep 12.
21. MicroRNA-222-3p associated with Helicobacter pylori targets HIPK2 to promote cell proliferation, invasion, and inhibits apoptosis in gastric cancer. Tan X, etal., J Cell Biochem. 2018 Jul;119(7):5153-5162. doi: 10.1002/jcb.26542. Epub 2018 Mar 25.
22. MicroRNA-221/222 Family Counteracts Myocardial Fibrosis in Pressure Overload-Induced Heart Failure. Verjans R, etal., Hypertension. 2018 Feb;71(2):280-288. doi: 10.1161/HYPERTENSIONAHA.117.10094. Epub 2017 Dec 18.
23. Role and mechanism of miR-222 in arsenic-transformed cells for inducing tumor growth. Wang M, etal., Oncotarget. 2016 Apr 5;7(14):17805-14. doi: 10.18632/oncotarget.7525.
24. MiR-222 overexpression confers cell migratory advantages in hepatocellular carcinoma through enhancing AKT signaling. Wong QW, etal., Clin Cancer Res. 2010 Feb 1;16(3):867-75. doi: 10.1158/1078-0432.CCR-09-1840. Epub 2010 Jan 26.
25. microRNA-222 promotes tumor growth and confers radioresistance in nasopharyngeal carcinoma by targeting PTEN. Wu W, etal., Mol Med Rep. 2018 Jan;17(1):1305-1310. doi: 10.3892/mmr.2017.7931. Epub 2017 Oct 31.
26. miR-221 and miR-222 expression increased the growth and tumorigenesis of oral carcinoma cells. Yang CJ, etal., J Oral Pathol Med. 2011 Aug;40(7):560-6. doi: 10.1111/j.1600-0714.2010.01005.x. Epub 2011 Jan 13.
27. MicroRNA-222-3p participates in the development of oral squamous cell carcinoma by targeting CDKN1B. Yang K, etal., J Oral Pathol Med. 2020 Aug;49(7):621-629. doi: 10.1111/jop.12986. Epub 2020 Mar 1.
28. Panax notoginseng saponins attenuate lung cancer growth in part through modulating the level of Met/miR-222 axis. Yang Q, etal., J Ethnopharmacol. 2016 Dec 4;193:255-265. doi: 10.1016/j.jep.2016.08.040. Epub 2016 Aug 24.
29. Downregulation of MicroRNA-222 Reduces Insulin Resistance in Rats with PCOS by Inhibiting Activation of the MAPK/ERK Pathway via Pten. Ye H, etal., Mol Ther Nucleic Acids. 2020 Jul 10;22:733-741. doi: 10.1016/j.omtn.2020.07.014. eCollection 2020 Dec 4.
30. Inhibition of microRNA-222 expression accelerates bone healing with enhancement of osteogenesis, chondrogenesis, and angiogenesis in a rat refractory fracture model. Yoshizuka M, etal., J Orthop Sci. 2016 Nov;21(6):852-858. doi: 10.1016/j.jos.2016.07.021. Epub 2016 Aug 18.
31. miR-221 and miR-222 promote Schwann cell proliferation and migration by targeting LASS2 after sciatic nerve injury. Yu B, etal., J Cell Sci. 2012 Jun 1;125(Pt 11):2675-83. doi: 10.1242/jcs.098996. Epub 2012 Mar 5.
32. [Expression of serum microRNAs (miR-222, miR-181, miR-216) in human hepatocellular carcinoma and its clinical significance]. Zhan MX, etal., Zhonghua Yi Xue Za Zhi. 2013 Jun 18;93(23):1830-2.
33. The alteration of miR-222 and its target genes in nickel-induced tumor. Zhang J, etal., Biol Trace Elem Res. 2013 May;152(2):267-74. doi: 10.1007/s12011-013-9619-6. Epub 2013 Feb 28.
34. Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1. Zhao H, etal., J Neuroinflammation. 2011 Nov 14;8:159. doi: 10.1186/1742-2094-8-159.
35. miR-222 regulates cell growth, apoptosis, and autophagy of interstitial cells of Cajal isolated from slow transit constipation rats by targeting c-kit. Zheng H, etal., Indian J Gastroenterol. 2021 Apr;40(2):198-208. doi: 10.1007/s12664-020-01143-7. Epub 2021 Apr 1.
36. MiR-21 and miR-222 inhibit apoptosis of adult dorsal root ganglion neurons by repressing TIMP3 following sciatic nerve injury. Zhou S, etal., Neurosci Lett. 2015 Jan 23;586:43-9. doi: 10.1016/j.neulet.2014.12.006. Epub 2014 Dec 4.
Additional References at PubMed
PMID:12624257   PMID:15634332   PMID:16381832   PMID:16682203   PMID:17369397   PMID:17604727   PMID:18452032   PMID:18723672   PMID:19109175   PMID:19147652   PMID:19188425   PMID:19559694  
PMID:19859555   PMID:20215419   PMID:20413612   PMID:20548288   PMID:20668074   PMID:20813046   PMID:21037258   PMID:21267068   PMID:21822254   PMID:21993888   PMID:22511778   PMID:22562984  
PMID:23185045   PMID:23221369   PMID:23409140   PMID:23632416   PMID:23637774   PMID:23646144   PMID:24030710   PMID:24308935   PMID:24498137   PMID:24569080   PMID:25086570   PMID:25858512  
PMID:26062936   PMID:26081516   PMID:26206211   PMID:26398571   PMID:26891296   PMID:27338035   PMID:28274991   PMID:28941704   PMID:29276985   PMID:29364977   PMID:30212751   PMID:30316865  
PMID:30669342   PMID:30826054   PMID:31126802   PMID:31312877   PMID:31389030   PMID:31637892   PMID:31770568   PMID:31894320   PMID:31923208   PMID:31941655   PMID:32457280   PMID:33657395  
PMID:34586363   PMID:34710568   PMID:34779712   PMID:35046889   PMID:35436629   PMID:36216078  


Comparative Map Data
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
GRCm39X19,013,132 - 19,013,210 (-)NCBIGRCm39GRCm39mm39
GRCm39 EnsemblX19,013,132 - 19,013,210 (-)EnsemblGRCm39 Ensembl
GRCm38X19,146,893 - 19,146,971 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 EnsemblX19,146,893 - 19,146,971 (-)EnsemblGRCm38mm10GRCm38
MGSCv37X18,724,019 - 18,724,097 (-)NCBIGRCm37MGSCv37mm9NCBIm37
CeleraX16,723,051 - 16,723,129 (-)NCBICelera
Cytogenetic MapXA1.3NCBI
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
GRCh38X45,747,015 - 45,747,124 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p13 EnsemblX45,747,015 - 45,747,124 (-)EnsemblGRCh38hg38GRCh38
GRCh37X45,606,421 - 45,606,530 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 36X45,491,364 - 45,491,473 (-)NCBINCBI36Build 36hg18NCBI36
CeleraX49,798,348 - 49,798,457 (-)NCBICelera
Cytogenetic MapXp11.3NCBI
HuRefX43,321,845 - 43,321,954 (-)NCBIHuRef
CHM1_1X45,638,880 - 45,638,989 (-)NCBICHM1_1
T2T-CHM13v2.0X45,156,474 - 45,156,583 (-)NCBIT2T-CHM13v2.0
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
mRatBN7.2X3,428,904 - 3,429,006 (+)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 EnsemblX3,428,904 - 3,429,006 (+)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_UtxX3,494,603 - 3,494,705 (+)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.0X6,970,399 - 6,970,501 (+)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.0X3,287,490 - 3,287,592 (+)NCBIRnor_WKY
Rnor_6.0X3,683,919 - 3,684,021 (+)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 EnsemblX3,683,919 - 3,684,021 (+)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.0X4,474,734 - 4,474,836 (+)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
CeleraX3,968,681 - 3,968,783 (+)NCBICelera
Cytogenetic MapXq11NCBI
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
CanFam3.1X39,524,519 - 39,524,608 (-)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 EnsemblX39,524,519 - 39,524,608 (-)EnsemblCanFam3.1canFam3CanFam3.1
Dog10K_Boxer_TashaX26,757,541 - 26,757,630 (-)NCBIDog10K_Boxer_Tasha
ROS_Cfam_1.0X39,656,443 - 39,656,532 (-)NCBIROS_Cfam_1.0
UMICH_Zoey_3.1X39,646,830 - 39,646,919 (-)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.0X39,633,847 - 39,633,936 (-)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.0X39,726,166 - 39,726,255 (-)NCBIUU_Cfam_GSD_1.0
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
Sscrofa11.1 EnsemblX40,571,808 - 40,571,887 (-)EnsemblSscrofa11.1susScr11Sscrofa11.1
Sscrofa11.1X40,571,808 - 40,571,887 (-)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2X45,275,613 - 45,275,692 (-)NCBISscrofa10.2Sscrofa10.2susScr3

miRNA Target Status

Confirmed Targets
Gene TargetMature miRNAMethod NameResult TypeData TypeSupport TypePMID
Kitmmu-miR-222-3pMirtarbaseexternal_infoLuciferase reporter assay//Microarray//qRT-PCRFunctional MTI16330772

Predicted Targets
Summary Value
Count of predictions:15685
Count of gene targets:7606
Count of transcripts:12056
Interacting mature miRNAs:mmu-miR-222-3p, mmu-miR-222-5p
Prediction methods:Microtar, Miranda, Pita, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.

QTLs in Region (GRCm39)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1301551Spha1_msperm head anomaly 1 (mouse)Not determinedX1026622539921652Mouse
1302195Pbwg10_mpostnatal body weight growth 10 (mouse)Not determinedX126202173Mouse
1357433Dbts2_mdiabetes 2 (mouse)Not determinedX7226295150107038Mouse
13208553Wght11_mweight 11 (mouse)X1186623959045360Mouse
13208557Lgth13_mbody length 13 (mouse)X1186623959045360Mouse



Reference Sequences
RefSeq Acc Id: ENSMUST00000083537
RefSeq Status:
Mouse AssemblyChrPosition (strand)Source
GRCm39 EnsemblX19,013,132 - 19,013,210 (-)Ensembl
GRCm38.p6 EnsemblX19,146,893 - 19,146,971 (-)Ensembl
RefSeq Acc Id: NR_029807
Mouse AssemblyChrPosition (strand)Source
GRCm39X19,013,132 - 19,013,210 (-)NCBI
GRCm38X19,146,893 - 19,146,971 (-)ENTREZGENE
MGSCv37X18,724,019 - 18,724,097 (-)RGD
CeleraX16,723,051 - 16,723,129 (-)RGD
RGD ID:13680160
Promoter ID:EPDNEW_M24229
Type:single initiation site
Description:Mus musculus microRNA 222 , microRNA.
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Mouse AssemblyChrPosition (strand)Source
GRCm38X19,146,897 - 19,146,957EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene MGI:3619118 AgrOrtholog
Ensembl Genes ENSMUSG00000065471 Ensembl, ENTREZGENE
Ensembl Transcript ENSMUST00000083537 ENTREZGENE
miRBase MI0000710 ENTREZGENE
PhenoGen Mir222 PhenoGen
RNAcentral URS00002D6573 RNACentral
  URS000075A2FD RNACentral
  URS0000D4F78E RNACentral