Mob3b (MOB kinase activator 3B) - Rat Genome Database

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Gene: Mob3b (MOB kinase activator 3B) Rattus norvegicus
Analyze
Symbol: Mob3b
Name: MOB kinase activator 3B
RGD ID: 1560696
Description: Predicted to enable protein kinase activator activity. Predicted to be involved in positive regulation of protein phosphorylation; regulation of hippo signaling; and signal transduction. Predicted to be active in cytoplasm and nucleus. Orthologous to human MOB3B (MOB kinase activator 3B); INTERACTS WITH 6-propyl-2-thiouracil; amphetamine; bisphenol A.
Type: protein-coding
RefSeq Status: VALIDATED
Previously known as: LOC102550026; LOC366352; MOB kinase activator 3B-like; MOB1, Mps One Binder kinase activator-like 2B; MOB1, Mps One Binder kinase activator-like 2B (yeast); Mobkl2b; mps one binder kinase activator-like 2B; RGD1560696; similar to Mob3b protein; uncharacterized LOC102550026
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Dog
Squirrel
Pig
Naked Mole-Rat
Alliance Genes
More Info more info ...
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
mRatBN7.2549,541,804 - 49,737,934 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl549,545,815 - 49,737,934 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx551,739,475 - 51,931,145 (-)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.0553,339,724 - 53,531,407 (-)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.0553,241,656 - 53,433,259 (-)NCBIRnor_WKY
Rnor_6.0550,437,444 - 50,638,136 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl550,438,576 - 50,638,136 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.0555,006,398 - 55,201,845 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.4551,616,232 - 51,809,856 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
Celera548,289,837 - 48,481,822 (-)NCBICelera
Cytogenetic Map5q21NCBI
JBrowse: View Region in Genome Browser (JBrowse)
Model


Gene-Chemical Interaction Annotations     Click to see Annotation Detail View
Gene Ontology Annotations     Click to see Annotation Detail View

Biological Process

Cellular Component
cytoplasm  (IBA)
nucleus  (IBA)

Molecular Function

References

References - curated
# Reference Title Reference Citation
1. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
2. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
Additional References at PubMed
PMID:28792927  


Genomics

Comparative Map Data
Mob3b
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
mRatBN7.2549,541,804 - 49,737,934 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl549,545,815 - 49,737,934 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx551,739,475 - 51,931,145 (-)NCBIRnor_SHR
UTH_Rnor_SHRSP_BbbUtx_1.0553,339,724 - 53,531,407 (-)NCBIRnor_SHRSP
UTH_Rnor_WKY_Bbb_1.0553,241,656 - 53,433,259 (-)NCBIRnor_WKY
Rnor_6.0550,437,444 - 50,638,136 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl550,438,576 - 50,638,136 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.0555,006,398 - 55,201,845 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.4551,616,232 - 51,809,856 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
Celera548,289,837 - 48,481,822 (-)NCBICelera
Cytogenetic Map5q21NCBI
MOB3B
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh38927,325,209 - 27,529,814 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p13 Ensembl927,325,209 - 27,529,814 (-)EnsemblGRCh38hg38GRCh38
GRCh37927,325,207 - 27,529,812 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 36927,315,207 - 27,519,850 (-)NCBINCBI36Build 36hg18NCBI36
Build 34927,315,207 - 27,519,850NCBI
Celera927,253,352 - 27,457,971 (-)NCBICelera
Cytogenetic Map9p21.2NCBI
HuRef927,277,716 - 27,482,639 (-)NCBIHuRef
CHM1_1927,324,996 - 27,529,642 (-)NCBICHM1_1
T2T-CHM13v2.0927,335,803 - 27,540,380 (-)NCBIT2T-CHM13v2.0
Mob3b
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm39434,949,074 - 35,157,490 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl434,949,074 - 35,157,484 (-)EnsemblGRCm39 Ensembl
GRCm38434,949,074 - 35,157,524 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl434,949,074 - 35,157,484 (-)EnsemblGRCm38mm10GRCm38
MGSCv37434,899,953 - 35,104,715 (-)NCBIGRCm37MGSCv37mm9NCBIm37
MGSCv36435,141,591 - 35,346,353 (-)NCBIMGSCv36mm8
MGSCv36434,609,498 - 34,815,906 (-)NCBIMGSCv36mm8
Celera434,682,511 - 34,873,721 (-)NCBICelera
Cytogenetic Map4A5NCBI
cM Map416.86NCBI
Mob3b
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0 EnsemblNW_0049554727,425,819 - 7,530,719 (+)EnsemblChiLan1.0
ChiLan1.0NW_0049554727,381,248 - 7,535,818 (+)NCBIChiLan1.0ChiLan1.0
MOB3B
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
PanPan1.1927,778,739 - 27,983,880 (-)NCBIpanpan1.1PanPan1.1panPan2
PanPan1.1 Ensembl927,784,106 - 27,909,354 (-)Ensemblpanpan1.1panPan2
Mhudiblu_PPA_v0927,172,685 - 27,378,196 (-)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
MOB3B
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
CanFam3.11145,456,049 - 45,574,034 (-)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 Ensembl1145,460,901 - 45,574,034 (-)EnsemblCanFam3.1canFam3CanFam3.1
Dog10K_Boxer_Tasha1144,132,551 - 44,323,212 (-)NCBIDog10K_Boxer_Tasha
ROS_Cfam_1.01146,401,892 - 46,592,947 (-)NCBIROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl1146,405,651 - 46,592,952 (-)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.11145,057,960 - 45,248,366 (-)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.01144,948,160 - 45,145,340 (-)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.01145,694,768 - 45,885,793 (-)NCBIUU_Cfam_GSD_1.0
Mob3b
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2NW_024404947160,352,109 - 160,543,161 (-)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_0049366112,125,069 - 2,312,698 (+)EnsemblSpeTri2.0
SpeTri2.0NW_0049366112,125,249 - 2,312,943 (+)NCBISpeTri2.0SpeTri2.0SpeTri2.0
MOB3B
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1 Ensembl1038,438,346 - 38,680,995 (+)EnsemblSscrofa11.1susScr11Sscrofa11.1
Sscrofa11.11038,437,933 - 38,694,667 (+)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.21043,580,268 - 43,668,235 (+)NCBISscrofa10.2Sscrofa10.2susScr3
Mob3b
(Heterocephalus glaber - naked mole-rat)
Naked Mole-rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_00462473631,552,912 - 31,667,100 (-)EnsemblHetGla_female_1.0HetGla_female_1.0 EnsemblhetGla2
HetGla 1.0NW_00462473631,534,327 - 31,735,163 (-)NCBIHetGla_female_1.0HetGla 1.0hetGla2

Variants

.
Variants in Mob3b
1035 total Variants
miRNA Target Status

Predicted Target Of
Summary Value
Count of predictions:180
Count of miRNA genes:129
Interacting mature miRNAs:151
Transcripts:ENSRNOT00000012519
Prediction methods:Miranda, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (mRatBN7.2)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1331756Rf34Renal function QTL 344.16275kidney blood vessel physiology trait (VT:0100012)absolute change in renal blood flow rate (CMO:0001168)5190450412Rat
70212Niddm25Non-insulin dependent diabetes mellitus QTL 253.54blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)51131345958Rat
1331771Rf35Renal function QTL 354.36965kidney blood vessel physiology trait (VT:0100012)absolute change in renal blood flow rate (CMO:0001168)572947086724018Rat
1576314Eutr1Estrogen induced uterine response QTL 1uterus integrity trait (VT:0010575)pyometritis severity score (CMO:0002009)52138965166875058Rat
8662454Vetf3Vascular elastic tissue fragility QTL 327.4artery integrity trait (VT:0010639)number of ruptures of the internal elastic lamina of the abdominal aorta and iliac arteries (CMO:0002562)5228222669540447Rat
2293666Bmd38Bone mineral density QTL 384.4femur size trait (VT:1000369)femoral neck cortical cross-sectional area (CMO:0001702)5894822853948228Rat
7394712Emca13Estrogen-induced mammary cancer QTL 13mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)5982326699753708Rat
1641903Alcrsp3Alcohol response QTL 3response to alcohol trait (VT:0010489)duration of loss of righting reflex (CMO:0002289)51268928557689285Rat
634305Mamtr1Mammary tumor resistance QTL 10.0001mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)512789751113558310Rat
1600358Mamtr5Mammary tumor resistance QTL 5mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)51887394763873947Rat
1358353Srcrtb2Stress Responsive Cort Basal QTL 23.480.003blood corticosterone amount (VT:0005345)plasma corticosterone level (CMO:0001173)51887394774251464Rat
8552954Pigfal14Plasma insulin-like growth factor 1 level QTL 149blood insulin-like growth factor amount (VT:0010479)plasma insulin-like growth factor 1 level (CMO:0001299)52122674466226744Rat
1578767Stresp17Stress response QTL 174.30.01blood aldosterone amount (VT:0005346)plasma aldosterone level (CMO:0000551)52795544072955440Rat
1578776Stresp18Stress response QTL 182.9thymus mass (VT:0004954)thymus wet weight (CMO:0000855)52795544072955440Rat
6903292Stl28Serum triglyceride level QTL 282.60.0073blood triglyceride amount (VT:0002644)plasma triglyceride level (CMO:0000548)52851548973515489Rat
6903306Scl35Serum cholesterol QTL 352.60.0073blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)52851548973515489Rat
2290448Scl54Serum cholesterol level QTL 542.93blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)531663789131345958Rat
1598807Glom12Glomerulus QTL 122.7kidney glomerulus morphology trait (VT:0005325)index of glomerular damage (CMO:0001135)53321566578215665Rat
1298067Scl15Serum cholesterol level QTL 154.80.001blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)53321566578215665Rat
1302786Kidm8Kidney mass QTL 828.15kidney mass (VT:0002707)both kidneys wet weight to body weight ratio (CMO:0000340)53321566578215665Rat
1576317Eutr2Estrogen induced uterine response QTL 20.01uterus integrity trait (VT:0010575)pyometritis severity score (CMO:0002009)534730116104251008Rat
2316959Gluco59Glucose level QTL 594.7blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)534944474113558310Rat
1641922Alcrsp8Alcohol response QTL 8alcohol metabolism trait (VT:0015089)blood ethanol level (CMO:0000535)53518915368564008Rat
1641912Alcrsp18Alcohol response QTL 18response to alcohol trait (VT:0010489)duration of loss of righting reflex (CMO:0002289)535189153141643988Rat
1549845Scl44Serum cholesterol level QTL 446blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)540128307148607290Rat
1331773Scl26Serum cholesterol level QTL 263.065blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)54372665686724018Rat
1331801Rf33Renal function QTL 334.149kidney blood vessel physiology trait (VT:0100012)absolute change in renal vascular resistance (CMO:0001900)543726656129132602Rat
1578766Tcas11Tongue tumor susceptibility QTL 114.12tongue integrity trait (VT:0010553)number of squamous cell tumors of the tongue with diameter greater than 3 mm (CMO:0001950)546711509161317411Rat
1300115Hrtrt7Heart rate QTL 72.76heart pumping trait (VT:2000009)heart rate (CMO:0000002)54786906290099692Rat
9589025Epfw7Epididymal fat weight QTL 720.660.001epididymal fat pad mass (VT:0010421)epididymal fat pad weight to body weight ratio (CMO:0000658)54946360094463600Rat
7411561Bw134Body weight QTL 134240.001body mass (VT:0001259)body weight gain (CMO:0000420)54946360094463600Rat

Markers in Region
D5Rat254  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2549,593,783 - 49,593,924 (+)MAPPERmRatBN7.2
Rnor_6.0551,197,517 - 51,197,657NCBIRnor6.0
Rnor_6.0550,486,539 - 50,486,679NCBIRnor6.0
Rnor_5.0555,757,348 - 55,757,488UniSTSRnor5.0
Rnor_5.0555,055,493 - 55,055,633UniSTSRnor5.0
RGSC_v3.4551,664,209 - 51,664,350RGDRGSC3.4
RGSC_v3.4551,664,210 - 51,664,350UniSTSRGSC3.4
RGSC_v3.1551,664,388 - 51,664,529RGD
Celera548,337,575 - 48,337,715UniSTS
FHH x ACI Map529.14UniSTS
FHH x ACI Map529.14RGD
Cytogenetic Map5q21-q22UniSTS
BE105239  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.21079,246,664 - 79,247,408 (+)MAPPERmRatBN7.2
mRatBN7.2549,654,204 - 49,654,403 (+)MAPPERmRatBN7.2
Rnor_6.0550,552,703 - 50,552,901NCBIRnor6.0
Rnor_6.01081,997,248 - 81,997,991NCBIRnor6.0
Rnor_5.01081,830,061 - 81,830,804UniSTSRnor5.0
Rnor_5.0555,119,460 - 55,119,658UniSTSRnor5.0
RGSC_v3.41082,936,187 - 82,936,930UniSTSRGSC3.4
RGSC_v3.4551,725,332 - 51,725,530UniSTSRGSC3.4
Celera548,398,023 - 48,398,221UniSTS
Celera1078,035,466 - 78,036,209UniSTS
RH 3.4 Map5269.7UniSTS
Cytogenetic Map5q21-q22UniSTS
Cytogenetic Map10q26UniSTS
Cytogenetic Map5q22UniSTS


Expression


RNA-SEQ Expression
High: > 1000 TPM value   Medium: Between 11 and 1000 TPM
Low: Between 0.5 and 10 TPM   Below Cutoff: < 0.5 TPM

alimentary part of gastrointestinal system circulatory system endocrine system exocrine system hemolymphoid system hepatobiliary system integumental system musculoskeletal system nervous system renal system reproductive system respiratory system appendage
High
Medium 4 4 1 4 4 12 2
Low 3 31 53 37 18 37 6 8 70 23 35 11 6
Below cutoff 12 2 3 4 2

Sequence


Reference Sequences
RefSeq Acc Id: ENSRNOT00000012519   ⟹   ENSRNOP00000012519
RefSeq Status:
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl549,545,815 - 49,737,934 (-)Ensembl
Rnor_6.0 Ensembl550,438,576 - 50,638,136 (-)Ensembl
RefSeq Acc Id: ENSRNOT00000080713
RefSeq Status:
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.0 Ensembl551,148,371 - 51,184,078 (-)Ensembl
RefSeq Acc Id: NM_001108970   ⟹   NP_001102440
RefSeq Status: VALIDATED
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2549,545,815 - 49,737,934 (-)NCBI
Rnor_6.0550,438,576 - 50,638,136 (-)NCBI
Rnor_5.0555,006,398 - 55,201,845 (-)NCBI
RGSC_v3.4551,616,232 - 51,809,856 (-)RGD
Celera548,289,837 - 48,481,822 (-)RGD
Sequence:
RefSeq Acc Id: XM_006238001   ⟹   XP_006238063
RefSeq Status:
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2549,541,804 - 49,737,934 (-)NCBI
Rnor_6.0550,437,444 - 50,638,136 (-)NCBI
Rnor_5.0555,006,398 - 55,201,845 (-)NCBI
Sequence:
Protein Sequences
Protein RefSeqs NP_001102440 (Get FASTA)   NCBI Sequence Viewer  
  XP_006238063 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein EDL98612 (Get FASTA)   NCBI Sequence Viewer  
Reference Sequences
RefSeq Acc Id: NP_001102440   ⟸   NM_001108970
- UniProtKB: D3ZCN1 (UniProtKB/TrEMBL)
- Sequence:
RefSeq Acc Id: XP_006238063   ⟸   XM_006238001
- Peptide Label: isoform X1
- UniProtKB: D3ZCN1 (UniProtKB/TrEMBL)
- Sequence:
RefSeq Acc Id: ENSRNOP00000012519   ⟸   ENSRNOT00000012519

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-D3ZCN1-F1-model_v2 AlphaFold D3ZCN1 1-216 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13693624
Promoter ID:EPDNEW_R4149
Type:multiple initiation site
Name:Mob3b_1
Description:MOB kinase activator 3B
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.0550,638,149 - 50,638,209EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:1560696 AgrOrtholog
BioCyc Gene G2FUF-41642 BioCyc
Ensembl Genes ENSRNOG00000009453 Ensembl, ENTREZGENE, UniProtKB/TrEMBL
  ENSRNOG00000058323 Ensembl
Ensembl Protein ENSRNOP00000012519 ENTREZGENE
  ENSRNOP00000012519.3 UniProtKB/TrEMBL
Ensembl Transcript ENSRNOT00000012519 ENTREZGENE
  ENSRNOT00000012519.4 UniProtKB/TrEMBL
Gene3D-CATH 1.20.140.30 UniProtKB/TrEMBL
InterPro Mob1_phocein UniProtKB/TrEMBL
  MOB_kinase_act_sf UniProtKB/TrEMBL
KEGG Report rno:366352 UniProtKB/TrEMBL
NCBI Gene 366352 ENTREZGENE
PANTHER Mob1_phocein UniProtKB/TrEMBL
Pfam Mob1_phocein UniProtKB/TrEMBL
PhenoGen Mob3b PhenoGen
SMART Mob1_phocein UniProtKB/TrEMBL
Superfamily-SCOP Mob1_phocein UniProtKB/TrEMBL
UniProt D3ZCN1 ENTREZGENE, UniProtKB/TrEMBL


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2021-03-09 Mob3b  MOB kinase activator 3B  LOC102550026  uncharacterized LOC102550026  Data Merged 737654 PROVISIONAL
2013-12-17 LOC102550026  uncharacterized LOC102550026      Symbol and Name status set to provisional 70820 PROVISIONAL
2011-10-18 Mob3b  MOB kinase activator 3B  Mobkl2b  MOB1, Mps One Binder kinase activator-like 2B (yeast)  Nomenclature updated to reflect human and mouse nomenclature 1299863 APPROVED
2008-02-28 Mobkl2b  MOB1, Mps One Binder kinase activator-like 2B (yeast)  RGD1560696_predicted  similar to Mob3b protein (predicted)  Nomenclature updated to reflect human and mouse nomenclature 1299863 APPROVED
2006-03-07 RGD1560696_predicted  similar to Mob3b protein (predicted)  LOC366352  similar to Mob3b protein  Symbol and Name status set to approved 1299863 APPROVED
2006-02-09 LOC366352  similar to Mob3b protein      Symbol and Name status set to provisional 70820 PROVISIONAL