Cell Line : CVCL_KZ58 (PathHunter HEK 293 IkappaB Degradation) Homo sapiens
Symbol:
CVCL_KZ58
Name:
PathHunter HEK 293 IkappaB Degradation
RGD ID:
21144786
Cell Line Type:
transformed cell line
Origin:
Transformant: Adenovirus 5(NCBI-Taxonomy; 28285); Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Gender:
Female
Characteristics:
Characteristics: PathHunter degradation cell lines express a target protein tagged with a beta-galactosidase fragment (Enzyme Donor=ED) Pathway activation results in the degradation of the target protein. The degraded protein is detected by adding to the cell lysis buffer another beta-galactosidase fragment (Enzyme Acceptor=EA). Complementation of the ED and EA enzyme fragments result into a catalytically active enzyme that generate a chemiluminescent signal.