angiotensin II (3 ng/kg/min) (for 3 days) then physiological salt solution perfusate

 controlled tempol content drinking water (15 mg/kg/d) (for 3 days) then physiological salt solution perfusate then NG-nitroarginine methyl ester (100 umol/l) (for 0.3 hours)

 controlled sodium content diet (0.4 %) (between 70 and 84 days) then physiological salt solution perfusate

 angiotensin II (3 ng/kg/min) (for 3 days) then physiological salt solution perfusate then NG-nitroarginine methyl ester (100 umol/l) (for 0.3 hours)

 physiological salt solution perfusate

 0.9% sodium chloride solution (for 3 days) then physiological salt solution perfusate

 controlled sodium content diet (0.4 %) (between 70 and 84 days) then controlled sodium content diet (4 %) (for 6 days) then angiotensin II (100 ng/kg/min) (for 3 days) then physiological salt solution perfusate

 controlled tempol content drinking water (15 mg/kg/d) (for 3 days) then physiological salt solution perfusate

 controlled captopril content drinking water (100 mg/kg/d) (for 3 days) then physiological salt solution perfusate

 physiological salt solution perfusate then NG-nitroarginine methyl ester (100 umol/l) (for 0.3 hours)

 controlled losartan content drinking water (20 mg/kg/d) (for 7 days) then physiological salt solution perfusate

 controlled sodium content diet (0.4 %) (between 70 and 84 days) then controlled sodium content diet (4 %) (for 6 days) then vehicle control condition (for 3 days) then physiological salt solution perfusate