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Term:
o-Phthalaldehyde fluorescent protein assay (MMO:0000730)
Annotations: Rat: (0) Mouse: (0) Human: (0) Chinchilla: (0) Bonobo: (0) Dog: (0) Squirrel: (0) Pig: (0) Naked Mole-rat: (0) Green Monkey: (0)
Parent Terms Term With Siblings Child Terms
fluorometry +     
fluorimetric Morgan-Elson assay for hyaluronidase activity 
Fura-2 intracellular calcium assay  
o-Phthalaldehyde fluorescent protein assay 
When reacted with primary amines in the presence of mercaptoethanol, OPA (o-Phthalaldehyde) yields an intense blue-colored fluorescent product that has a maximum wavelength of excitation of 340 nm and emission at 455 nm. Wavelengths from 330-375 nm have been used for excitation and 436-490 nm for measuring emission. Protein concentrations as low as 50 ng/ml can be measured with an OPA assay. The inherent sensitivity and speed of OPA, along with its broad linear range, makes it a useful protein and peptide assay reagent.

Synonyms
Exact Synonyms: OPA fluorescent protein assay
Definition Sources: http://www.piercenet.com, PMID:5576608

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