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Ontology Browser

Term:
reverse transcription polymerase chain reaction assay (MMO:0000655)
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Parent Terms Term With Siblings Child Terms
real-time polymerase chain reaction assay +   
reverse transcription polymerase chain reaction assay +  
A method which assesses transcript expression by conversion of an RNA template into complementary DNA (cDNA) by reverse transcription. The cDNA is used as a template for exponential amplification by the polymerase chain reaction, a technique which produces a large number of copies of one or more specific DNA sequences via multiple rounds of thermal cycling where DNA is melted, annealed to oligonucleotide primers, and extended using a thermostable DNA polymerase.

Synonyms
Exact Synonyms: reverse transcription polymerase chain reaction
Related Synonyms: RT-PCR
Alternate IDs: MMO:0000461
Xrefs: ECO:0000109 ;   OBI:0001170 ;   OBI:0001361 ;   efo:EFO_0002943
display_synonym: RT-PCR
Definition Sources: https://en.wikipedia.org/wiki/Polymerase_chain_reaction "Wikipedia" "Wikipedia", https://en.wikipedia.org/wiki/Reverse_transcription_polymerase_chain_reaction "Wikipedia" "Wikipedia"

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