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Term:
real-time polymerase chain reaction assay (MMO:0000460)
Annotations: Rat: (5) Mouse: (0) Human: (0) Chinchilla: (0) Bonobo: (0) Dog: (0) Squirrel: (0) Pig: (0) Naked Mole-rat: (0) Green Monkey: (0)
Parent Terms Term With Siblings Child Terms
real-time polymerase chain reaction assay +   
Real-time PCR is able to detect sequence-specific PCR products as they accumulate in 'real-time' during the PCR amplification process. As the PCR product of interest is produced, real-time PCR can detect their accumulation and quantify the number of substrates present in the initial PCR mixture before amplification began.
reverse transcription polymerase chain reaction assay +  

Synonyms
Exact Synonyms: qPCR ;   quantitative real time polymerase chain reaction ;   real-time PCR
Related Synonyms: kinetic polymerase chain reaction
Definition Sources: http://www.bio.davidson.edu/courses/genomics/method/realtimepcr.html

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