bicinchoninic acid protein assay - Ontology Browser

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bicinchoninic acid protein assay (MMO:0000424)
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protein analysis by liquid phase colorimetry with spectrophotometric readout + is-a

automated bromocresol green-based plasma albumin analysis with spectrophotometric readout

bicinchoninic acid protein assay

The BCA protein assay combines the protein-induced biuret reaction in which peptide bonds in the protein reduce Cu2+ ions to Cu+ in a reaction proportional to the amount of protein present, followed by chelation of bicinchoninic acid molecules with Cu+ ions, forming a purple-colored product which can be quantitated by spectrophotometry, i.e., based on the ability of the solution to alter the transmission of light at specific wavelengths in the visible spectrum, that is, to alter the transmission of a specific color of light.

Bradford protein assay

bromocresol green-based urine albumin analysis with spectrophotometric readout

Lowry protein assay

Synonyms
Related Synonyms:	BCA protein assay
Definition Sources:	Dorland:Dorlands_Illustrated_Medical_Dictionary--31st_Ed, http://en.wikipedia.org/wiki/Bicinchoninic_acid_assay "Wikipedia" "Wikipedia", http://www.piercenet.com/browse.cfm?fldID=876562B0-5056-8A76-4E0C-B764EAB3A339 "Website" "Website", ISBN:978-1416049982, McGraw-Hill:McGraw-Hill_Dictionary_of_Scientific_and_Technical_Terms--4th_Ed

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