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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (http://rgd.mcw.edu/contact/index.shtml).

Term:polymerase chain reaction assay
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Accession:MMO:0000459 term browser browse the term
Definition:A rapid technique for in vitro amplification of specific DNA sequences, allowing small quantities of short sequences to be analyzed without cloning. DNA sequences are amplified in vitro by separating the DNA into two strands and incubating it in a series of temperature cycles with oligonucleotide primers and heat-stable DNA polymerase.
Synonyms:exact_synonym: PCR


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  measurement method 0
    ex vivo method 0
      enzymatic method 0
        polymerase chain reaction assay 0
          real-time polymerase chain reaction assay + 0
          reverse transcription polymerase chain reaction assay + 0
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  measurement method 0
    molecular method 0
      expression assay 0
        transcript expression assay 0
          polymerase chain reaction assay 0
            real-time polymerase chain reaction assay + 0
            reverse transcription polymerase chain reaction assay + 0
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