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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (http://rgd.mcw.edu/contact/index.shtml).
Term:Bradford protein assay
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Accession:MMO:0000426 term browser browse the term
Definition:The Bradford assay of protein concentration is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. In the acidic environment of the assay, protein binds to the Coomassie dye, resulting in a shift from reddish/brown to blue in the color of the dye, which can be quantitated by spectrophotometry, i.e., based on the ability of the solution to alter the transmission of light at specific wavelengths in the visible spectrum, that is, to alter the transmission of a specific color of light. The binding of the dye to the protein stabilizes the blue anionic form and the increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample.
Synonyms:exact_synonym: Coomassie dye protein assay


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Related Phenotype Data for Term "Bradford protein assay" (MMO:0000426)

Rat Strains:
Clinical Measurements:
Experimental Conditions:
Measurement Methods:
Term paths to the root
Path 1
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        spectrophotometry 21
          protein analysis by liquid phase colorimetry with spectrophotometric readout 9
            Bradford protein assay 0
Path 2
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        photometry 40
          colorimetry 40
            liquid phase colorimetry 36
              peptide/protein analysis by liquid phase colorimetry 10
                protein analysis by liquid phase colorimetry with spectrophotometric readout 9
                  Bradford protein assay 0
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