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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (http://rgd.mcw.edu/contact/index.shtml).
Term:Lowry protein assay
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Accession:MMO:0000425 term browser browse the term
Definition:The Lowry assay is an enhanced biuret test involving copper chelation chemistry to assess the amount of protein, any of a group of complex organic compounds containing carbon, hydrogen, oxygen, nitrogen, and sulfur consisting of alpha-amino acids joined by peptide linkages, in a specified sample. Following the Folin-Ciocalteu reaction, the reduced Folin reagent is quantitated by spectrophotometry, i.e., based on the ability of the solution to alter the transmission of light at specific wavelengths in the visible spectrum, that is, to alter the transmission of a specific color of light.


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Lowry protein assay term browser
Symbol Object Name Evidence Notes Source PubMed Reference(s) RGD Reference(s) Position
Q Aocep1 Aortic cell protein QTL 1 IDA RGD PMID:15668357 RGD:1334448 NCBI chr17:40,990,005...85,990,005 JBrowse link
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Path 1
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        spectrophotometry 21
          protein analysis by liquid phase colorimetry with spectrophotometric readout 9
            Lowry protein assay 1
Path 2
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        photometry 40
          colorimetry 40
            liquid phase colorimetry 36
              peptide/protein analysis by liquid phase colorimetry 10
                protein analysis by liquid phase colorimetry with spectrophotometric readout 9
                  Lowry protein assay 1
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