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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (http://rgd.mcw.edu/contact/index.shtml).
Term:Fura-2 intracellular calcium assay
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Accession:MMO:0000252 term browser browse the term
Definition:Method which employs Fura-2 or a related compound such as Fura 2-AM, its membrane-permeable derivative, to measure the amount or concentration of calcium inside isolated cells. Output is determined by fluorometry.


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Fura-2 intracellular calcium assay term browser
Symbol Object Name Evidence Notes Source PubMed Reference(s) RGD Reference(s) Position
Q Calcic1 Intracellular calcium level QTL 1 IDA RGD PMID:15882549 RGD:1547855 NCBI chr12:28,064,433...40,130,419 JBrowse link
Q Calcic2 Intracellular calcium level QTL 2 IDA RGD PMID:17261463 RGD:1599588 NCBI chr12:28,064,433...46,669,029 JBrowse link

Related Phenotype Data for Term "Fura-2 intracellular calcium assay" (MMO:0000252)

Rat Strains:
Clinical Measurements:
Experimental Conditions:
Measurement Methods:
Term paths to the root
Path 1
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        spectrophotometry 21
          fluorometry 3
            Fura-2 intracellular calcium assay 2
Path 2
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      isolated cell method 14
        cell-based biologic assay 9
          intracellular calcium assay 2
            fluorescent intracellular calcium assay 2
              Fura-2 intracellular calcium assay 2
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