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CHEBI ONTOLOGY - ANNOTATIONS

The Chemical Entities of Biological Interest (ChEBI) ontology is downloaded weekly from EMBL-EBI at http://www.ebi.ac.uk/chebi/. The data is made available under the Creative Commons License (CC BY 3.0, http://creativecommons.org/licenses/by/3.0/). For more information see: Degtyarenko et al. (2008) ChEBI: a database and ontology for chemical entities of biological interest. Nucleic Acids Res. 36, D344–D350.

Term:fluorescein 5-isothiocyanate
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Accession:CHEBI:37918 term browser browse the term
Definition:The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
Synonyms:exact_synonym: 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
 related_synonym: 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)-5-isothiocyanatobenzoic acid;   3',6'-dihydroxy-5-isothiocyanatospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one;   5-FITC;   5-isothiocyanatofluorescein;   F5ITC;   FITC isomer 1;   FITC-I;   Fluorescein-5-isothiocyanat;   Fluoreszein-5-isothiocyanat;   Formula=C21H11NO5S;   InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H;   InChIKey=MHMNJMPURVTYEJ-UHFFFAOYSA-N;   SMILES=Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(ccc23)N=C=S)c1;   fluorescein isothiocyanate isomer 1
 xref: Beilstein:1407295;   CAS:3326-32-7
 xref_mesh: MESH:D016650
 xref: PMID:11122447;   PMID:19583962;   PMID:19709338;   PMID:19768221;   PMID:20492570;   PMID:21300019;   PMID:21451573;   PMID:21456254;   PMID:22009769;   PMID:22718031;   PMID:23426683;   PMID:23667843;   PMID:25515858;   PMID:25602940;   PMID:25703728;   PMID:27373998;   PMID:28972093;   Patent:GB2467901;   Reaxys:1407295


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fluorescein 5-isothiocyanate term browser
Symbol Object Name Qualifiers Evidence Notes Source PubMed Reference(s) RGD Reference(s) Position
G Abcb7 ATP binding cassette subfamily B member 7 affects binding ISO Fluorescein-5-isothiocyanate binds to ABCB7 protein CTD PMID:27129696 NCBI chr  X:75,150,511...75,291,950
Ensembl chr  X:75,150,608...75,291,938
JBrowse link
G Akr1c2 aldo-keto reductase family 1, member C2 increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of AKR1C2 mRNA CTD PMID:20491607 NCBI chr17:69,388,337...69,435,160
Ensembl chr17:69,388,335...69,404,341
JBrowse link
G Anxa4 annexin A4 affects binding ISO Fluorescein-5-isothiocyanate binds to ANXA4 protein CTD PMID:27129696 NCBI chr 4:118,538,775...118,595,591
Ensembl chr 4:118,538,777...118,595,580
JBrowse link
G Arhgap6 Rho GTPase activating protein 6 affects binding ISO Fluorescein-5-isothiocyanate binds to ARHGAP6 protein CTD PMID:27129696 NCBI chr  X:26,314,561...26,845,242
Ensembl chr  X:26,315,878...26,376,467
JBrowse link
G Azi2 5-azacytidine induced 2 affects binding ISO Fluorescein-5-isothiocyanate binds to AZI2 protein CTD PMID:27129696 NCBI chr 8:126,411,030...126,435,581
Ensembl chr 8:126,411,829...126,434,579
JBrowse link
G Capzb capping actin protein of muscle Z-line subunit beta affects binding ISO Fluorescein-5-isothiocyanate binds to CAPZB protein CTD PMID:27129696 NCBI chr 5:157,642,742...157,742,487
Ensembl chr 5:157,642,751...157,742,478
JBrowse link
G Cd109 CD109 molecule affects binding ISO Fluorescein-5-isothiocyanate binds to CD109 protein CTD PMID:27129696 NCBI chr 8:85,951,420...86,065,411
Ensembl chr 8:85,951,191...86,065,409
JBrowse link
G Cd180 CD180 molecule affects binding ISO Fluorescein-5-isothiocyanate binds to CD180 protein CTD PMID:27129696 NCBI chr 2:32,820,275...32,833,223
Ensembl chr 2:32,820,322...32,833,125
JBrowse link
G Cd86 CD86 molecule increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of CD86 protein CTD PMID:19665512 NCBI chr11:67,060,305...67,117,990
Ensembl chr11:67,082,193...67,118,795
JBrowse link
G Cd93 CD93 molecule affects binding ISO Fluorescein-5-isothiocyanate binds to CD93 protein CTD PMID:27129696 NCBI chr 3:142,778,798...142,783,774
Ensembl chr 3:142,778,770...142,783,774
JBrowse link
G Cdc20 cell division cycle 20 affects binding ISO Fluorescein-5-isothiocyanate binds to CDC20 protein CTD PMID:27129696 NCBI chr 5:137,260,728...137,264,931
Ensembl chr 5:137,260,739...137,265,015
JBrowse link
G Copa COPI coat complex subunit alpha affects binding ISO Fluorescein-5-isothiocyanate binds to COPA protein CTD PMID:27129696 NCBI chr13:90,467,285...90,508,894
Ensembl chr13:90,467,265...90,508,932
JBrowse link
G Cyp1a1 cytochrome P450, family 1, subfamily a, polypeptide 1 increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of CYP1A1 mRNA CTD PMID:20491607 NCBI chr 8:62,472,087...62,478,122
Ensembl chr 8:62,472,095...62,478,147
JBrowse link
G Dcaf4 DDB1 and CUL4 associated factor 4 affects binding ISO Fluorescein-5-isothiocyanate binds to DCAF4 protein CTD PMID:27129696 NCBI chr 6:107,001,763...107,027,466
Ensembl chr 6:107,001,763...107,027,456
JBrowse link
G Dot1l DOT1 like histone lysine methyltransferase affects binding ISO Fluorescein-5-isothiocyanate binds to DOT1L protein CTD PMID:27129696 NCBI chr 7:11,786,105...11,824,742
Ensembl chr 7:11,786,105...11,824,742
JBrowse link
G Elac1 elaC ribonuclease Z 1 affects binding ISO Fluorescein-5-isothiocyanate binds to ELAC1 protein CTD PMID:27129696 NCBI chr18:69,706,522...69,723,869
Ensembl chr18:69,706,522...69,723,869
JBrowse link
G Enpp7 ectonucleotide pyrophosphatase/phosphodiesterase 7 affects binding ISO Fluorescein-5-isothiocyanate binds to ENPP7 protein CTD PMID:27129696 NCBI chr10:108,095,131...108,099,483
Ensembl chr10:108,095,131...108,099,483
JBrowse link
G Flrt3 fibronectin leucine rich transmembrane protein 3 affects binding ISO Fluorescein-5-isothiocyanate binds to FLRT3 protein CTD PMID:27129696 NCBI chr 3:134,684,446...134,696,586
Ensembl chr 3:134,683,065...134,696,654
JBrowse link
G Fn1 fibronectin 1 affects binding ISO Fluorescein-5-isothiocyanate binds to FN1 protein CTD PMID:27129696 NCBI chr 9:78,900,111...78,969,018
Ensembl chr 9:78,900,103...78,969,078
JBrowse link
G Hmox1 heme oxygenase 1 increases expression
multiple interactions
ISO Fluorescein-5-isothiocyanate results in increased expression of HMOX1 protein
[Melatonin co-treated with diisodecyl phthalate co-treated with Fluorescein-5-isothiocyanate] results in increased expression of HMOX1 protein
CTD PMID:27871981 NCBI chr19:14,508,634...14,515,455
Ensembl chr19:14,508,616...14,515,456
JBrowse link
G Hspa5 heat shock protein family A (Hsp70) member 5 affects binding ISO Fluorescein-5-isothiocyanate binds to HSPA5 protein CTD PMID:27129696 NCBI chr 3:13,838,304...13,842,763
Ensembl chr 3:13,838,304...13,842,762
JBrowse link
G Hspa9 heat shock protein family A (Hsp70) member 9 affects binding ISO Fluorescein-5-isothiocyanate binds to HSPA9 protein CTD PMID:27129696 NCBI chr18:27,731,072...27,749,235
Ensembl chr18:27,731,072...27,749,235
JBrowse link
G Ifng interferon gamma multiple interactions ISO [Dibutyl Phthalate co-treated with Fluorescein-5-isothiocyanate] results in increased secretion of IFNG protein; [tributyrin co-treated with Fluorescein-5-isothiocyanate] results in increased secretion of IFNG protein CTD PMID:29198882 NCBI chr 7:61,337,383...61,341,419
Ensembl chr 7:61,337,381...61,341,419
JBrowse link
G Ikbkb inhibitor of nuclear factor kappa B kinase subunit beta affects binding ISO Fluorescein-5-isothiocyanate binds to IKBKB protein CTD PMID:27129696 NCBI chr16:74,177,233...74,230,809
Ensembl chr16:74,177,215...74,230,815
JBrowse link
G Ikzf4 IKAROS family zinc finger 4 affects binding ISO Fluorescein-5-isothiocyanate binds to IKZF4 protein CTD PMID:27129696 NCBI chr 7:3,061,422...3,098,142
Ensembl chr 7:3,061,469...3,074,359
JBrowse link
G Il17re interleukin 17 receptor E affects binding ISO Fluorescein-5-isothiocyanate binds to IL17RE protein CTD PMID:27129696 NCBI chr 4:145,413,157...145,426,608
Ensembl chr 4:145,413,230...145,426,603
JBrowse link
G Il31ra interleukin 31 receptor A affects binding ISO Fluorescein-5-isothiocyanate binds to IL31RA protein CTD PMID:27129696 NCBI chr 2:44,333,347...44,408,005
Ensembl chr 2:44,336,006...44,394,709
JBrowse link
G Il4 interleukin 4 multiple interactions
increases expression
ISO [Dibutyl Phthalate co-treated with Fluorescein-5-isothiocyanate] results in increased secretion of IL4 protein; [dibutyrylglycerol analog co-treated with Fluorescein-5-isothiocyanate] affects the secretion of IL4 protein; [tributyrin co-treated with Fluorescein-5-isothiocyanate] results in increased secretion of IL4 protein; diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased expression of IL4 protein]; Melatonin inhibits the reaction [diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased expression of IL4 protein]] CTD PMID:27871981, PMID:29198882 NCBI chr10:38,963,979...38,969,531
Ensembl chr10:38,963,979...38,969,531
JBrowse link
G Lypd3 Ly6/Plaur domain containing 3 affects binding ISO Fluorescein-5-isothiocyanate binds to LYPD3 protein CTD PMID:27129696 NCBI chr 1:81,499,856...81,504,306
Ensembl chr 1:81,499,821...81,504,318
JBrowse link
G Maff MAF bZIP transcription factor F increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of MAFF mRNA CTD PMID:20491607 NCBI chr 7:120,580,743...120,592,095
Ensembl chr 7:120,580,743...120,592,095
JBrowse link
G Map3k20 mitogen-activated protein kinase kinase kinase 20 affects binding ISO Fluorescein-5-isothiocyanate binds to MAP3K20 protein CTD PMID:27129696 NCBI chr 3:58,965,025...59,120,507
Ensembl chr 3:58,965,552...59,067,369
JBrowse link
G Mapk1 mitogen activated protein kinase 1 increases phosphorylation
multiple interactions
ISO Fluorescein-5-isothiocyanate results in increased phosphorylation of MAPK1 protein
Fluorescein-5-isothiocyanate inhibits the reaction [Dinitrofluorobenzene results in increased phosphorylation of MAPK1 protein]; Fluorescein-5-isothiocyanate inhibits the reaction [Lipopolysaccharides results in increased phosphorylation of MAPK1 protein]
CTD PMID:27129696 NCBI chr11:88,203,863...88,273,301
Ensembl chr11:88,211,599...88,273,254
JBrowse link
G Mapk3 mitogen activated protein kinase 3 increases phosphorylation
multiple interactions
ISO Fluorescein-5-isothiocyanate results in increased phosphorylation of MAPK3 protein
Fluorescein-5-isothiocyanate inhibits the reaction [Dinitrofluorobenzene results in increased phosphorylation of MAPK3 protein]; Fluorescein-5-isothiocyanate inhibits the reaction [Lipopolysaccharides results in increased phosphorylation of MAPK3 protein]
CTD PMID:27129696 NCBI chr 1:198,192,773...198,198,975
Ensembl chr 1:198,192,773...198,198,975
JBrowse link
G Mapk8 mitogen-activated protein kinase 8 multiple interactions
increases phosphorylation
ISO Fluorescein-5-isothiocyanate inhibits the reaction [Dinitrofluorobenzene results in increased phosphorylation of MAPK8 protein]; Fluorescein-5-isothiocyanate inhibits the reaction [Lipopolysaccharides results in increased phosphorylation of MAPK8 protein]
Fluorescein-5-isothiocyanate results in increased phosphorylation of MAPK8 protein
CTD PMID:27129696 NCBI chr16:9,620,854...9,709,342
Ensembl chr16:9,625,177...9,709,347
JBrowse link
G Mapk9 mitogen-activated protein kinase 9 multiple interactions
increases phosphorylation
ISO Fluorescein-5-isothiocyanate inhibits the reaction [Dinitrofluorobenzene results in increased phosphorylation of MAPK9 protein]; Fluorescein-5-isothiocyanate inhibits the reaction [Lipopolysaccharides results in increased phosphorylation of MAPK9 protein]
Fluorescein-5-isothiocyanate results in increased phosphorylation of MAPK9 protein
CTD PMID:27129696 NCBI chr10:35,333,859...35,374,364
Ensembl chr10:35,333,859...35,374,355
JBrowse link
G Mt2A metallothionein 2A increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of MT2A mRNA CTD PMID:20491607 NCBI chr19:11,307,966...11,308,740
Ensembl chr19:11,307,967...11,308,740
JBrowse link
G Mybpc1 myosin binding protein C1 affects binding ISO Fluorescein-5-isothiocyanate binds to MYBPC1 protein CTD PMID:27129696 NCBI chr 7:29,086,159...29,171,909
Ensembl chr 7:29,086,156...29,171,783
JBrowse link
G Nfe2l2 nuclear factor, erythroid 2-like 2 multiple interactions ISO [Melatonin co-treated with diisodecyl phthalate co-treated with Fluorescein-5-isothiocyanate] results in increased expression of NFE2L2 protein CTD PMID:27871981 NCBI chr 3:62,497,568...62,525,146
Ensembl chr 3:62,497,571...62,524,996
JBrowse link
G Nqo1 NAD(P)H quinone dehydrogenase 1 increases expression
multiple interactions
ISO Fluorescein-5-isothiocyanate results in increased expression of NQO1 mRNA
Fluorescein-5-isothiocyanate results in increased expression of NQO1 protein
diisodecyl phthalate inhibits the reaction [Fluorescein-5-isothiocyanate results in increased expression of NQO1 protein]; Melatonin inhibits the reaction [diisodecyl phthalate inhibits the reaction [Fluorescein-5-isothiocyanate results in increased expression of NQO1 protein]]
CTD PMID:20491607, PMID:27871981 NCBI chr19:38,422,210...38,437,103
Ensembl chr19:38,422,164...38,437,180
JBrowse link
G Plce1 phospholipase C, epsilon 1 affects binding ISO Fluorescein-5-isothiocyanate binds to PLCE1 protein CTD PMID:27129696 NCBI chr 1:257,156,023...257,465,440
Ensembl chr 1:257,157,264...257,466,064
JBrowse link
G Postn periostin affects binding ISO Fluorescein-5-isothiocyanate binds to POSTN protein CTD PMID:27129696 NCBI chr 2:143,656,820...143,688,087
Ensembl chr 2:143,656,793...143,688,087
JBrowse link
G Pprc1 PPARG related coactivator 1 affects binding ISO Fluorescein-5-isothiocyanate binds to PPRC1 protein CTD PMID:27129696 NCBI chr 1:265,809,892...265,826,319
Ensembl chr 1:265,809,754...265,826,306
JBrowse link
G Prkcb protein kinase C, beta increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of PRKCB protein CTD PMID:31958493 NCBI chr 1:192,233,569...192,575,339
Ensembl chr 1:192,233,910...192,574,831
JBrowse link
G Psd4 pleckstrin and Sec7 domain containing 4 affects binding ISO Fluorescein-5-isothiocyanate binds to PSD4 protein CTD PMID:27129696 NCBI chr 3:1,478,432...1,515,094
Ensembl chr 3:1,478,525...1,514,934
JBrowse link
G Psmd14 proteasome 26S subunit, non-ATPase 14 affects binding ISO Fluorescein-5-isothiocyanate binds to PSMD14 protein CTD PMID:27129696 NCBI chr 3:47,578,449...47,673,330
Ensembl chr 3:47,578,384...47,673,338
JBrowse link
G Rela RELA proto-oncogene, NF-kB subunit multiple interactions ISO [diisodecyl phthalate co-treated with Fluorescein-5-isothiocyanate] results in increased phosphorylation of RELA protein; Melatonin inhibits the reaction [[diisodecyl phthalate co-treated with Fluorescein-5-isothiocyanate] results in increased phosphorylation of RELA protein] CTD PMID:27871981 NCBI chr 1:220,992,770...221,003,249
Ensembl chr 1:220,992,770...221,003,249
JBrowse link
G Scn5a sodium voltage-gated channel alpha subunit 5 affects binding ISO Fluorescein-5-isothiocyanate binds to SCN5A protein CTD PMID:27129696 NCBI chr 8:128,169,191...128,266,681
Ensembl chr 8:128,169,191...128,266,639
JBrowse link
G Skor1 SKI family transcriptional corepressor 1 affects binding ISO Fluorescein-5-isothiocyanate binds to SKOR1 protein CTD PMID:27129696 NCBI chr 8:68,029,930...68,040,504
Ensembl chr 8:68,030,621...68,038,533
JBrowse link
G Slc38a10 solute carrier family 38, member 10 affects binding ISO Fluorescein-5-isothiocyanate binds to SLC38A10 protein CTD PMID:27129696 NCBI chr10:109,284,054...109,333,907
Ensembl chr10:109,284,957...109,333,899
JBrowse link
G Stat3 signal transducer and activator of transcription 3 multiple interactions
increases phosphorylation
ISO diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT3 protein]; Melatonin inhibits the reaction [diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT3 protein]] CTD PMID:27871981 NCBI chr10:88,790,401...88,842,263
Ensembl chr10:88,790,408...88,842,233
JBrowse link
G Stat5a signal transducer and activator of transcription 5A multiple interactions
increases phosphorylation
ISO diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT5 protein]; Melatonin inhibits the reaction [diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT5 protein]] CTD PMID:27871981 NCBI chr10:88,764,732...88,789,060
Ensembl chr10:88,764,732...88,789,057
JBrowse link
G Stat6 signal transducer and activator of transcription 6 increases phosphorylation
multiple interactions
ISO Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT6 protein
diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT6 protein]; Melatonin inhibits the reaction [diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased phosphorylation of STAT6 protein]]
CTD PMID:27871981 NCBI chr 7:70,946,228...70,963,542
Ensembl chr 7:70,946,228...70,964,484
JBrowse link
G Trpa1 transient receptor potential cation channel, subfamily A, member 1 affects binding ISO Fluorescein-5-isothiocyanate binds to TRPA1 protein CTD PMID:27129696 NCBI chr 5:3,783,247...3,836,485
Ensembl chr 5:3,783,247...3,836,485
JBrowse link
G Tslp thymic stromal lymphopoietin increases expression
multiple interactions
ISO Fluorescein-5-isothiocyanate results in increased expression of TSLP protein
diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased expression of TSLP protein]; Melatonin inhibits the reaction [diisodecyl phthalate promotes the reaction [Fluorescein-5-isothiocyanate results in increased expression of TSLP protein]]
CTD PMID:27871981 NCBI chr18:25,613,601...25,618,066
Ensembl chr18:25,613,831...25,617,361
JBrowse link
G Txn1 thioredoxin 1 increases expression ISO Fluorescein-5-isothiocyanate results in increased expression of TXN mRNA CTD PMID:20491607 NCBI chr 5:75,049,735...75,057,731
Ensembl chr 5:75,049,747...75,057,752
JBrowse link
G Usp3 ubiquitin specific peptidase 3 affects binding ISO Fluorescein-5-isothiocyanate binds to USP3 protein CTD PMID:27129696 NCBI chr 8:72,207,970...72,284,871
Ensembl chr 8:72,207,972...72,284,873
JBrowse link

Term paths to the root
Path 1
Term Annotations click to browse term
  CHEBI ontology 19792
    role 19738
      biological role 19737
        antigen 114
          fluorescein isothiocyanate 56
            fluorescein 5-isothiocyanate 56
Path 2
Term Annotations click to browse term
  CHEBI ontology 19792
    subatomic particle 19788
      composite particle 19788
        hadron 19788
          baryon 19788
            nucleon 19788
              atomic nucleus 19788
                atom 19788
                  main group element atom 19675
                    p-block element atom 19675
                      carbon group element atom 19571
                        carbon atom 19560
                          organic molecular entity 19560
                            organic group 18508
                              organic divalent group 18501
                                organodiyl group 18501
                                  carbonyl group 18404
                                    carbonyl compound 18404
                                      carboxylic ester 14089
                                        lactone 5987
                                          gamma-lactone 1220
                                            fluorescein 67
                                              fluorescein isothiocyanate 56
                                                fluorescein 5-isothiocyanate 56
paths to the root

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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.