| URN | urn:agi-ProtModification:inout-urn:agi-llid:2869:out-urn:agi-llid:154::unknown |
|---|---|
| References | 7 |
| Connectivity | 2 |
| Effect | unknown |
| TextRef | info:pmid/8662852#abs:4 |
|---|---|
| MedLine Reference | 8662852:3 |
| Sentence | Here we report the identification of the sites of GRK2- and GRK5-mediated beta2AR phosphorylation. |
| TextRef | info:pmid/8170959#abs:8 |
|---|---|
| CellLineName | Sf9 |
| MedLine Reference | 8170959:7 |
| Sentence | While phosphorylation of the beta 2-AR by GRK2, -3, and -5 is similar, the activity of GRK2 and -3 is enhanced by G beta gamma whereas that of GRK5 is not. |
| TextRef | info:pmid/8120045#abs:6 |
|---|---|
| CellLineName | Sf9 |
| MedLine Reference | 8120045:5 |
| Sentence | Purified GRK5 phosphorylates rhodopsin in a light-dependent manner and beta 2-adrenergic receptor in an agonist-dependent manner and phosphorylates the C-terminal tail regions of both receptor proteins. |
| TextRef | info:pmid/10499588#abs:6 |
|---|---|
| MedLine Reference | 10499588:5 |
| Sentence | A serine residue at position 411 in the tail of the beta2-adrenergic receptor is a substrate for phosphorylation by GRK-5 (for G-protein-coupled-receptor kinase-5) and is required for interaction with EBP50 and for proper recycling of the receptor. |
| TextRef | info:pmid/8144599#abs:5 |
|---|---|
| MedLine Reference | 8144599:4 |
| Sentence | Expression and characterization of a mutant GRK5 that does not autophosphorylate (S484A and T485A) reveals that the mutant has a approximately 15-20-fold reduced ability to phosphorylate the beta 2-adrenergic receptor and rhodopsin compared to wild type GRK5. |
| TextRef | info:pmid/19092051#abs:5 |
|---|---|
| MedLine Reference | 19092051:4 |
| Sentence | However, tyrosyl phosphorylation of GRK5 was not necessary for GRK5-mediated phosphorylation of the beta(2)-adrenergic receptor, even though beta(2)-adrenergic receptor activation promoted tyrosyl phosphorylation of GRK5 in smooth muscle cells. |
| Tissue | smooth muscle |
| TextRef | info:pmid/8288567#abs:4 |
|---|---|
| MedLine Reference | 8288567:3 |
| Sentence | GRK5 phosphorylates the beta 2-adrenergic receptor (beta 2AR), m2 muscarinic cholinergic receptor, and rhodopsin in an agonist-dependent manner to maximal stoichiometries of approximately 2.5, 1.5, and 1 mol of phosphate/mol of receptor, respectively, with Km values of approximately 0.5 microM for the beta 2AR, approximately 16 microM for rhodopsin, and approximately 24 microM for ATP. |