Ariadne Genomics Inc. ERs coactivators ERs corepressors ERs chaperones G protein mediated signaling pathway via Galphas family membrane tethered ERs - rapid, non-genomic effects Esr1 ---> direct and indirect target gene expression Esr1 ---> direct and indirect target gene expression ERs corepressors ---| Esr2 ERs corepressors ---| Esr1 Src ---> epidermal growth factor/neuregulin signaling pathway Src G protein mediated signaling pathway via Galphas family --+> cAMP ERs coactivators --+> Esr1 ERs coactivators ---- Esr1 Esr2 ---> direct and indirect target gene expression direct and indirect target gene expression Esr2 Esr2 ---> direct and indirect target gene expression Esr1 ERs coactivators ---- Esr2 ERs corepressors ---- Esr2 ERs corepressors ---- Esr1 ERs coactivators --+> Esr2 ERs chaperones ---- Esr2 ERs chaperones ---- Esr1 Esr1 ---- estradiol Esr2 ---- estradiol estradiol ---- Gper cAMP G protein mediated signaling pathway via Galphas family --+> Src mERs estradiol ---- mERs mERs ---- Src Gper epidermal growth factor/neuregulin signaling pathway estradiol biosynthetic pathway estradiol