Elsevier Inc. Eif4a Eif4g Eef1a1 no-go mRNA decay pathway nonsense-mediated mRNA decay pathway nonstop mRNA decay pathway Met Met. Etf1 ---> peptidyl-tRNA hydrolysis GTPt EIf5b 60S subunit ---- EIf5b 48S IC ---- 60S subunit 60S subunit 48S IC 80S ribosome IC mRNA scanning Dhx29 ---> 43S PIC 43S PIC Eif4a ---- Eif4g Eif4g ---- Pabpc1 Pabpc1 stop codon recognition peptide release peptidyl-tRNA hydrolysis stalled 80S Eef2 translocation PIC formation Pelo ---- stalled 80S ribosome recyling subunit dissociation aa3. aa2. aa3 aa2 deacylated-tRNA peptidyl-tRNA aa-tRNA cognate-tRNA mRNA structure ribosome biogenesis pathway translation initiation pathway aminoacyl-tRNA biosynthetic pathway mRNA nuclear export pathway spliceosome pathway Eif1a ---- EIf5b Eif2 trimer ---- Eif2b pentamer Eif5 Met-tRNA cog-tRNAe POST-translocation peptidyl-tRNA ---> deacylated-tRNA GTPe. ---> GDPe. PRE-translocation ---> POST-translocation GDP ---> GTP Eif2b pentamer Eif2 trimer EIF3 complex Eif1a Eif1 Hbs1l Eef1b2 Eif4h --+> Eif4a Eif4b Dhx29 Eif4e Eif4h GTP ---> GDP 48S IC ---- EIf5b Eif4b --+> Eif4a ribosome biogenesis pathway ---> translation initiation pathway cognate-tRNA ---> cog-tRNAe GSPT1 ---- GTPt GDPe ---> GTPe aminoacyl-tRNA biosynthetic pathway ---> aa-tRNA aa-tRNA ---- Eef1a1 Eef1a1 ---- GDPe Eef1a1 ---- GTPe Eif2 trimer ---- Met-tRNA aminoacyl-tRNA biosynthetic pathway ---> Met-tRNA Eif2 trimer ---- Eif5 EIF3 complex ---- Eif4g 40S subunit ---- Eif1 40S subunit ---- Eif2 trimer 40S subunit ---- Eif1a 40S subunit ---- Eif5 40S subunit ---- EIF3 complex Eif4e ---- Eif4g Eif2 trimer ---- GDP aa3 ---> aa2 mRNA nuclear export pathway ---> Eif4e spliceosome pathway ---> mRNA nuclear export pathway 80S ribosome IC ---- EIf5b Hbs1l ---- Pelo Etf1 ---- stop codon recognition mRNA structure ---- Pelo no stop codon ---- Pelo translation elongation pathway translation termination pathway GTPe ---> GDPe Eif2 trimer ---- GTP Eef2 ---- GTPe. PRE-translocation Eef2 ---- GDPe. Abce1 ---- stalled 80S Pelo Abce1 ---- Pelo Abce1 ---> subunit dissociation Abce1 Etf1 GDPt ---- GSPT1 GTPt ---> GDPt Etf1 ---- GSPT1 Abce1 ---- Etf1 GSPT1 --+> peptide release 40S subunit ---> 43S PIC GSPT1 GTP GTPe GTPe. GDP GDPe. GDPe GDPt 40S subunit premature stop codon no stop codon Etf1 ---- premature stop codon