Identification Systems

• Implantable transponders
  • Bio Medic Data Systems
  • Kent Scientific
• Ear notching
  • Harvard Apparatus
• Ear tagging
  • Harvard Apparatus
• Tattoos
  • Animal Identification & Marking System
  • Harvard Apparatus
    

Genotyping Protocols

• PhysGen Genotyping protocols for consomic development

DNA Extraction
Flourescent Genotyping PCR
PCR Mix Macro
Pooling and Purifying

• What anesthetics affect the GABA-ergic system?
• What are the dose of hormones and timing needed for inducing superovulation in rat females?
• How do you differentiate the sex of neonatal rats?
• What is a sensitive ELISA kit for detecting estradiol and testosterone in the blood of rats?

What anesthetics affect the GABA-ergic system?
GABA-ergic mechanisms are affected by barbiturates, benzodiazepines, propofol and steriod anesthetics and indirectly by volatile (gas) anesthetics. Some researchers have found success with Avertin(tribomoethanol).

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What are the dose of hormones and timing needed for inducing superovulation in rat females?
20 IU PMSG and 30 IU HCG 48 hours apart has been used successfully. See Filipiak WE, Saunders TL 2006, Advances in transgenic rat production. Transgenic Res. 15:673-686.

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How do you differentiate the sex of neonatal rats?
One way is to examine the ano-genital distance which will be longer in males than females.

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What is a sensitive ELISA kit for detecting estradiol and testosterone in the blood of rats?
Diagnostic Products Corps's Coat-A-Count testosterone RIA kit (marketed for human blood/urine) has been used by some groups with success. For estradiol, Caymen Chemical's Estradioal EIA kit has been suggested.

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