For years, researchers have used mice despite the fact that in many cases rat is a better model because the technology for producing rat knockouts simply didn't exist. That is no longer the case. These videos give a brief introduction to using Zinc Finger Nucleases to introduce germline mutations into targeted locations in the rat genome. The first video is an excerpt from an interview with Dr. Howard Jacob, the director of the Human and Molecular Genetics Center at the Medical College of Wisconsin. Dr. Jacob addresses how the recent development of targeted mutagenesis in rats will help develop better models for disease, physiology and pharmacology research.The second video gives an animated overview of how the Zinc Finger Nuclease technology works to produce targeted mutations, gene knockouts and gene knock-ins.
For more information, click here to access the CompozrZFN website.
For Zinc Finger Nuclease FAQs, click here.
Researchers at the Human and Molecular Genetics Center at the Medical College of Wisconsin are currently using this technology to knock out genes in the rat and published their results in the July24, 2009 issue of Science: Gurts et al, Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases. Science. 2009 Jul 24;325(5939):433. PMID: 19628861
Click here to view the article on the Science website (includes supplemental materials)
Or click here to download a pdf file of the paper.