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Strain Registration

Strain: WI-ROSA26em1(CAG-EGFP)Kyo

Symbol: WI-ROSA26em1(CAG-EGFP)Kyo
Strain: WI-ROSA26em1(CAG-EGFP)
Substrain: Kyo
RGD ID: 11040977
Citation ID: RRID:RGD_11040977
Ontology ID: RS:0004162
Also Known As: W-Rosa26em1(CAG-EGFP)Kyo; Rosa EGFP knockin; NBRP Rat No: 0770; WI-ROSA26em1(CAG-EGFP)Kyo; WI-ROSA26^[em1(CAG-EGFP)Kyo]
Type: mutant
Available Source: National BioResource Project for the Rat in Japan
Origination: National BioResource Project for the Rat in Japan
Description: CAG-GFP vector was knock-in into the rat ROSA26 locus by CRISPR/Cas9 system. Background strain: Crlj:WI. ROSA26 is a synonym for rat Thumpd3-as1 (RGD:6491660) and is used as an official symbol for rat strain nomenclature.
Last Known Status: Cryopreserved Sperm (as of 2017-08-08)






References

References - curated
# Reference Title Reference Citation
1. Strains registered by the National Bio Resource Project for the Rat in Japan Personal Communication with NBRP
2. RGD Strain RSO annotation pipeline RGD Automated Pipelines
3. ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Yoshimi K, etal., Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.

Region


Additional Information

RGD Curation Notes
Note Type Note Reference
strain_characteristics Strong and stable systemic EGFP expression under CAG-promoter in the rat Rosa26 locus 1302502
strain_reproduction maintained in EGFP-homozygous condition 1302502

Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2017-08-08 WI-ROSA26em1(CAG-EGFP)Kyo    WI-Rosa26em1(CAG-EGFP)Kyo    Symbol updated 68687 APPROVED
2017-08-08 WI-ROSA26em1(CAG-EGFP)Kyo    WI-Rosa26em1(CAG-EGFP)Kyo    Symbol updated 68687 APPROVED