Npff (neuropeptide FF-amide peptide precursor) - Rat Genome Database

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Gene: Npff (neuropeptide FF-amide peptide precursor) Rattus norvegicus
Analyze
Symbol: Npff
Name: neuropeptide FF-amide peptide precursor
RGD ID: 62033
Description: Enables G protein-coupled receptor binding activity and neuropeptide hormone activity. Involved in several processes, including negative regulation of appetite; negative regulation of insulin secretion; and peptide hormone secretion. Located in several cellular components, including axon terminus; dendrite; and perikaryon. Is active in neuronal dense core vesicle. Orthologous to human NPFF (neuropeptide FF-amide peptide precursor); INTERACTS WITH 6-propyl-2-thiouracil; amitrole; bisphenol A.
Type: protein-coding
RefSeq Status: VALIDATED
Previously known as: FMRFamide-related peptides; pro-FMRFamide-related neuropeptide FF
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Squirrel
Pig
Green Monkey
Naked Mole-Rat
Black Rat
Alliance Orthologs
More Info more info ...
Latest Assembly: GRCr8 - GRCr8 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr87135,533,050 - 135,533,839 (-)NCBIGRCr8GRCr8GRCr8
GRCr8 Ensembl7135,533,051 - 135,533,839 (-)EnsemblGRCr8
mRatBN7.27133,654,530 - 133,655,319 (-)NCBImRatBN7.2mRatBN7.2
UTH_Rnor_SHR_Utx7135,410,275 - 135,411,064 (-)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.07137,639,642 - 137,640,431 (-)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.07137,625,895 - 137,626,684 (-)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.07144,127,084 - 144,127,873 (-)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl7144,127,085 - 144,127,873 (-)Ensemblrn6Rnor6.0
Rnor_5.07141,919,079 - 141,919,868 (-)NCBIRnor_5.0Rnor_5.0rn5
Celera7130,082,133 - 130,082,891 (-)NCBICelera
Cytogenetic Map7q36NCBI
JBrowse:




Disease Annotations     Click to see Annotation Detail View
Inflammation  (IEP)

Gene Ontology Annotations     Click to see Annotation Detail View

Cellular Component

Molecular Function

References

References - curated
# Reference Title Reference Citation
1. Centrally administered neuropeptide FF inhibits arginine vasopressin release in conscious rats. Arima H, etal., Endocrinology. 1996 May;137(5):1523-9.
2. Identification and characterization of two G protein-coupled receptors for neuropeptide FF. Bonini JA, etal., J Biol Chem 2000 Dec 15;275(50):39324-31.
3. Synaptic actions of neuropeptide FF in the rat parabrachial nucleus: interactions with opioid receptors. Chen X, etal., J Neurophysiol. 2000 Aug;84(2):744-51.
4. Changes in neuropeptide FF and NPY immunohistochemical patterns in rat brain under heroin treatment. D'Este L, etal., Brain Res. 2006 Apr 14;1083(1):151-8. Epub 2006 Mar 10.
5. Release of neuropeptide FF, an anti-opioid peptide, in rat spinal cord slices is voltage- and Ca(2+)-sensitive: possible involvement of P-type Ca2+ channels. Devillers JP, etal., J Neurochem. 1995 Apr;64(4):1567-75.
6. A novel cryptic peptide derived from the rat neuropeptide FF precursor reverses antinociception and conditioned place preference induced by morphine. Dylag T, etal., Peptides. 2008 Mar;29(3):473-8. Epub 2007 Sep 29.
7. The effects of two FMRFamide related peptides (A-18-F-amide and F-8-F-amide; 'morphine modulating peptides') on the endocrine and exocrine rat pancreas. Fehmann HC, etal., Neuropeptides. 1990 Oct;17(2):87-92.
8. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
9. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
10. Central administration of neuropeptide FF (NPFF) causes increased neuronal activation and up-regulation of NPFF gene expression in the rat brainstem. Jhamandas JH and Mactavish D, J Comp Neurol 2002 Jun 3;447(3):300-7.
11. Neuropeptide FF and neuropeptide VF inhibit GABAergic neurotransmission in parvocellular neurons of the rat hypothalamic paraventricular nucleus. Jhamandas JH, etal., Am J Physiol Regul Integr Comp Physiol. 2007 May;292(5):R1872-80. Epub 2007 Feb 8.
12. Neuropeptide FF, but not prolactin-releasing peptide, mRNA is differentially regulated in the hypothalamic and medullary neurons after salt loading. Kalliomaki ML and Panula P, Neuroscience 2004;124(1):81-7.
13. Ultrastructural localization of neuropeptide FF, a new neuropeptide in the brain and pituitary of rats. Kivipelto L Regul Pept. 1991 Jul 9;34(3):211-24.
14. Cardiovascular effects induced by the stimulation of neuropeptide FF receptors in the dorsal vagal complex: an autoradiographic and pharmacological study in the rat. Laguzzi R, etal., Brain Res. 1996 Mar 4;711(1-2):193-202.
15. Rat ISS GO annotations from MGI mouse gene data--August 2006 MGD data from the GO Consortium
16. Neuropeptide FF reduces food intake in rats. Murase T, etal., Peptides. 1996;17(2):353-4.
17. Electronic Transfer of LocusLink and RefSeq Data NCBI rat LocusLink and RefSeq merged data July 26, 2002
18. Expression of neuropeptide FF, prolactin-releasing peptide, and the receptor UHR1/GPR10 genes during embryogenesis in the rat. Nieminen ML, etal., Dev Dyn 2003 Mar;226(3):561-9.
19. Pain- and morphine-associated transcriptional regulation of neuropeptide FF and the G-protein-coupled NPFF2 receptor gene. Nystedt JM, etal., Neurobiol Dis. 2004 Jun;16(1):254-62.
20. GOA pipeline RGD automated data pipeline
21. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
22. Tentative Sequence Identification Numbers Tentative Sequence Data IDs. TIGR Gene Index, Rat Data
23. Gene for pain modulatory neuropeptide NPFF: induction in spinal cord by noxious stimuli. Vilim FS, etal., Mol Pharmacol 1999 May;55(5):804-11.
Additional References at PubMed
PMID:11587714   PMID:12668052   PMID:14557236   PMID:16517015   PMID:18295932   PMID:22342307   PMID:24316399   PMID:25247577   PMID:28825666   PMID:33255594  


Genomics

Comparative Map Data
Npff
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr87135,533,050 - 135,533,839 (-)NCBIGRCr8GRCr8GRCr8
GRCr8 Ensembl7135,533,051 - 135,533,839 (-)EnsemblGRCr8
mRatBN7.27133,654,530 - 133,655,319 (-)NCBImRatBN7.2mRatBN7.2
UTH_Rnor_SHR_Utx7135,410,275 - 135,411,064 (-)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.07137,639,642 - 137,640,431 (-)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.07137,625,895 - 137,626,684 (-)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.07144,127,084 - 144,127,873 (-)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl7144,127,085 - 144,127,873 (-)Ensemblrn6Rnor6.0
Rnor_5.07141,919,079 - 141,919,868 (-)NCBIRnor_5.0Rnor_5.0rn5
Celera7130,082,133 - 130,082,891 (-)NCBICelera
Cytogenetic Map7q36NCBI
NPFF
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh381253,506,688 - 53,507,484 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 Ensembl1253,506,684 - 53,507,491 (-)Ensemblhg38GRCh38
GRCh371253,900,472 - 53,901,268 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 361252,186,741 - 52,187,689 (-)NCBIBuild 36Build 36hg18NCBI36
Build 341252,186,740 - 52,187,689NCBI
Celera1253,550,832 - 53,551,780 (-)NCBICelera
Cytogenetic Map12q13.13NCBI
HuRef1250,940,056 - 50,941,004 (-)NCBIHuRef
CHM1_11253,867,530 - 53,868,478 (-)NCBICHM1_1
T2T-CHM13v2.01253,472,693 - 53,473,489 (-)NCBIT2T-CHM13v2.0
Npff
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm3915102,432,274 - 102,433,056 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl15102,432,274 - 102,433,377 (-)EnsemblGRCm39 EnsemblGRCm39
GRCm3815102,523,839 - 102,524,621 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl15102,523,839 - 102,524,942 (-)Ensemblmm10GRCm38
MGSCv3715102,354,270 - 102,355,373 (-)NCBIMGSCv37MGSCv37mm9NCBIm37
MGSCv3615102,351,873 - 102,352,655 (-)NCBIMGSCv36mm8
Celera15104,681,604 - 104,682,707 (-)NCBICelera
Cytogenetic Map15F3NCBI
cM Map1557.65NCBI
Npff
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0 EnsemblNW_004955458799,072 - 799,745 (-)Ensembl
ChiLan1.0NW_004955458799,072 - 799,745 (-)NCBIChiLan1.0ChiLan1.0
NPFF
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
NHGRI_mPanPan1-v21040,677,155 - 40,677,958 (+)NCBINHGRI_mPanPan1-v2
NHGRI_mPanPan11240,673,919 - 40,674,722 (+)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v01235,245,172 - 35,246,710 (+)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.11236,030,056 - 36,031,591 (+)NCBIPanPan1.1PanPan1.1panPan2
PanPan1.1 Ensembl1236,030,805 - 36,031,591 (+)EnsemblpanPan2panpan1.1
Npff
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2NW_02440494562,382,476 - 62,384,553 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_00493651210,780,354 - 10,780,930 (-)EnsemblSpeTri2.0 Ensembl
SpeTri2.0NW_00493651210,780,277 - 10,782,219 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
NPFF
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1 Ensembl518,723,784 - 18,724,826 (-)EnsemblsusScr11Sscrofa11.1
Sscrofa11.1518,724,078 - 18,724,844 (-)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2519,159,707 - 19,160,579 (-)NCBISscrofa10.2Sscrofa10.2susScr3
NPFF
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChlSab1.11149,607,352 - 49,608,304 (-)NCBIChlSab1.1ChlSab1.1chlSab2
ChlSab1.1 Ensembl1149,607,443 - 49,608,139 (-)EnsemblChlSab1.1 EnsemblchlSab2Vervet-AGM
Vero_WHO_p1.0NW_023666037196,445,746 - 196,446,698 (+)NCBIVero_WHO_p1.0Vero_WHO_p1.0
Npff
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_0046249041,603,743 - 1,605,519 (-)EnsemblHetGla_female_1.0 EnsemblhetGla2HetGla_female_1.0 Ensembl
HetGla 1.0NW_0046249041,603,743 - 1,606,141 (-)NCBIHetGla 1.0HetGla 1.0hetGla2
Npff
(Rattus rattus - black rat)
Black Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Rrattus_CSIRO_v11260,557,197 - 260,560,602 (+)NCBIRrattus_CSIRO_v1

miRNA Target Status (No longer updated)

Predicted Target Of
Summary Value
Count of predictions:87
Count of miRNA genes:76
Interacting mature miRNAs:81
Transcripts:ENSRNOT00000074497
Prediction methods:Microtar, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (GRCr8)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1354582Stl11Serum triglyceride level QTL 113.42blood triglyceride amount (VT:0002644)serum triglyceride level (CMO:0000360)7121393075137014596Rat
634331Pia17Pristane induced arthritis QTL 174.7joint integrity trait (VT:0010548)arthritic paw count (CMO:0001460)7132099939137014596Rat
731176Glom5Glomerulus QTL 52.50.0035kidney glomerulus morphology trait (VT:0005325)count of superficial glomeruli not directly contacting the kidney surface (CMO:0001002)798549867137014596Rat
1300112Bp183Blood pressure QTL 1833.51arterial blood pressure trait (VT:2000000)blood pressure time series experimental set point of the baroreceptor response (CMO:0002593)7119280025135560836Rat
61428Scl3Serum cholesterol level QTL 33.2blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)7100548246137014596Rat
61397Bw17Body weight QTL 176.3body mass (VT:0001259)body weight (CMO:0000012)795485047137014596Rat
1358914Bp266Blood pressure QTL 266arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)7115313999136544683Rat
1549899Stresp8Stress response QTL 84.370.0008stress-related behavior trait (VT:0010451)defensive burying duration (CMO:0001961)792362341137014596Rat
1358891Bp265Blood pressure QTL 2652.21arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)771827901136544683Rat
1357339Stl14Serum triglyceride level QTL 143.450.0001blood triglyceride amount (VT:0002644)serum triglyceride level (CMO:0000360)7135371291137014596Rat

Markers in Region
RH134482  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.27133,654,086 - 133,654,303 (+)MAPPERmRatBN7.2
Rnor_6.07144,126,641 - 144,126,857NCBIRnor6.0
Rnor_5.07141,918,636 - 141,918,852UniSTSRnor5.0
Celera7130,081,690 - 130,081,906UniSTS
RH 3.4 Map71051.41UniSTS
Cytogenetic Map7q36UniSTS


Expression

RNA-SEQ Expression

alimentary part of gastrointestinal system
appendage
circulatory system
ectoderm
endocrine system
endoderm
exocrine system
hemolymphoid system
hepatobiliary system
integumental system
mesenchyme
mesoderm
musculoskeletal system
nervous system
renal system
reproductive system
respiratory system
sensory system
visual system
15 12 62 153 90 89 58 85 58 6 338 176 7 136 81 92 31 14 14

Sequence


Ensembl Acc Id: ENSRNOT00000074497   ⟹   ENSRNOP00000065607
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.0 Ensembl7144,127,085 - 144,127,873 (-)Ensembl
Ensembl Acc Id: ENSRNOT00000144668   ⟹   ENSRNOP00000102833
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 Ensembl7135,533,051 - 135,533,839 (-)Ensembl
RefSeq Acc Id: NM_022586   ⟹   NP_072108
RefSeq Status: VALIDATED
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr87135,533,050 - 135,533,839 (-)NCBI
mRatBN7.27133,654,530 - 133,655,319 (-)NCBI
Rnor_6.07144,127,084 - 144,127,873 (-)NCBI
Rnor_5.07141,919,079 - 141,919,868 (-)NCBI
Celera7130,082,133 - 130,082,891 (-)RGD
Sequence:
Protein Sequences
Protein RefSeqs NP_072108 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein AAD39828 (Get FASTA)   NCBI Sequence Viewer  
  EDL86824 (Get FASTA)   NCBI Sequence Viewer  
  EDL86825 (Get FASTA)   NCBI Sequence Viewer  
Ensembl Protein ENSRNOP00000102833
GenBank Protein Q9WVA9 (Get FASTA)   NCBI Sequence Viewer  
RefSeq Acc Id: NP_072108   ⟸   NM_022586
- Peptide Label: preproprotein
- UniProtKB: Q9WVA9 (UniProtKB/Swiss-Prot),   A6KCW7 (UniProtKB/TrEMBL)
- Sequence:
Ensembl Acc Id: ENSRNOP00000065607   ⟸   ENSRNOT00000074497
Ensembl Acc Id: ENSRNOP00000102833   ⟸   ENSRNOT00000144668

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-Q9WVA9-F1-model_v2 AlphaFold Q9WVA9 1-114 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13695704
Promoter ID:EPDNEW_R6229
Type:single initiation site
Name:Npff_1
Description:neuropeptide FF-amide peptide precursor
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.07144,127,847 - 144,127,907EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:62033 AgrOrtholog
BioCyc Gene G2FUF-31931 BioCyc
Ensembl Genes ENSRNOG00000047739 Ensembl
  ENSRNOG00000074090 Ensembl, ENTREZGENE
Ensembl Transcript ENSRNOT00000144668 ENTREZGENE
InterPro NPFF UniProtKB/Swiss-Prot
KEGG Report rno:60337 UniProtKB/Swiss-Prot
NCBI Gene 60337 ENTREZGENE
PANTHER PRO-FMRFAMIDE-RELATED NEUROPEPTIDE FF UniProtKB/Swiss-Prot
  PTHR15044 UniProtKB/Swiss-Prot
Pfam NPFF UniProtKB/Swiss-Prot
PharmGKB NPFF RGD
PhenoGen Npff PhenoGen
PIRSF FMRFamid-rel_pep_precur UniProtKB/Swiss-Prot
PRINTS FMRFAMIDEPEP UniProtKB/Swiss-Prot
RatGTEx ENSRNOG00000047739 RatGTEx
  ENSRNOG00000074090 RatGTEx
TIGR TC222691
UniProt A6KCW7 ENTREZGENE, UniProtKB/TrEMBL
  A6KCW8_RAT UniProtKB/TrEMBL
  NPFF_RAT UniProtKB/Swiss-Prot, ENTREZGENE


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2004-09-10 Npff  neuropeptide FF-amide peptide precursor    neuropeptide FF-amide peptide precursor   Name updated 1299863 APPROVED
2002-06-10 Npff  neuropeptide FF-amide peptide precursor       Name updated 70584 APPROVED

RGD Curation Notes
Note Type Note Reference
gene_regulation capable of up-regulating its own gene expression in the brainstem nucleus of the solitary tract 633355