Mid2 (midline 2) - Rat Genome Database

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Pathways
Gene: Mid2 (midline 2) Rattus norvegicus
Analyze
Symbol: Mid2
Name: midline 2
RGD ID: 1565674
Description: Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including host-mediated suppression of symbiont invasion; negative regulation of viral transcription; and positive regulation of canonical NF-kappaB signal transduction. Predicted to be located in cytoplasm and cytoskeleton. Predicted to colocalize with microtubule. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2); INTERACTS WITH (+)-schisandrin B; 2,3,7,8-tetrachlorodibenzodioxine; amphetamine.
Type: protein-coding
RefSeq Status: VALIDATED
Previously known as: LOC363502; midline-2; probable E3 ubiquitin-protein ligase MID2; RGD1565674; similar to Trim1 alpha
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Dog
Squirrel
Pig
Green Monkey
Naked Mole-Rat
Black Rat
Alliance Orthologs
More Info more info ...
Latest Assembly: GRCr8 - GRCr8 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr8X109,143,057 - 109,245,257 (+)NCBIGRCr8GRCr8GRCr8
GRCr8 EnsemblX109,143,253 - 109,245,255 (+)EnsemblGRCr8
mRatBN7.2X104,354,692 - 104,456,757 (+)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 EnsemblX104,355,316 - 104,453,473 (+)EnsemblmRatBN7.2
UTH_Rnor_SHR_UtxX106,484,643 - 106,582,790 (+)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.0X109,983,989 - 110,082,138 (+)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.0X107,531,877 - 107,629,899 (+)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.0X112,019,897 - 112,121,980 (+)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 EnsemblX112,020,646 - 112,121,943 (+)Ensemblrn6Rnor6.0
Rnor_5.0352,479,569 - 52,581,663 (-)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.4X128,496,689 - 128,595,233 (+)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera343,484,990 - 43,583,167 (-)NCBICelera
Cytogenetic MapXq33NCBI
JBrowse:




Disease Annotations     Click to see Annotation Detail View

Gene Ontology Annotations     Click to see Annotation Detail View

Cellular Component
cytoskeleton  (IEA)
microtubule  (IBA,IEA,ISO)

Molecular Function

References

References - curated
# Reference Title Reference Citation
1. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
2. GOA pipeline RGD automated data pipeline
3. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
4. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
Additional References at PubMed
PMID:10400986   PMID:11806752   PMID:18248090   PMID:19056867   PMID:23077300   PMID:25416956   PMID:26347139  


Genomics

Comparative Map Data
Mid2
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr8X109,143,057 - 109,245,257 (+)NCBIGRCr8GRCr8GRCr8
GRCr8 EnsemblX109,143,253 - 109,245,255 (+)EnsemblGRCr8
mRatBN7.2X104,354,692 - 104,456,757 (+)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 EnsemblX104,355,316 - 104,453,473 (+)EnsemblmRatBN7.2
UTH_Rnor_SHR_UtxX106,484,643 - 106,582,790 (+)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.0X109,983,989 - 110,082,138 (+)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.0X107,531,877 - 107,629,899 (+)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.0X112,019,897 - 112,121,980 (+)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 EnsemblX112,020,646 - 112,121,943 (+)Ensemblrn6Rnor6.0
Rnor_5.0352,479,569 - 52,581,663 (-)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.4X128,496,689 - 128,595,233 (+)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera343,484,990 - 43,583,167 (-)NCBICelera
Cytogenetic MapXq33NCBI
MID2
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh38X107,825,735 - 107,931,637 (+)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 EnsemblX107,825,755 - 107,931,637 (+)Ensemblhg38GRCh38
GRCh37X107,068,965 - 107,174,867 (+)NCBIGRCh37GRCh37hg19GRCh37
Build 36X106,956,116 - 107,057,077 (+)NCBIBuild 36Build 36hg18NCBI36
CeleraX107,540,045 - 107,645,826 (+)NCBICelera
Cytogenetic MapXq22.3NCBI
HuRefX96,692,632 - 96,797,758 (+)NCBIHuRef
CHM1_1X106,979,937 - 107,085,716 (+)NCBICHM1_1
T2T-CHM13v2.0X106,262,471 - 106,368,728 (+)NCBIT2T-CHM13v2.0
Mid2
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm39X139,565,326 - 139,668,464 (+)NCBIGRCm39GRCm39mm39
GRCm39 EnsemblX139,565,348 - 139,668,464 (+)EnsemblGRCm39 EnsemblGRCm39
GRCm38X140,664,304 - 140,767,715 (+)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 EnsemblX140,664,599 - 140,767,715 (+)Ensemblmm10GRCm38
MGSCv37X137,212,567 - 137,302,254 (+)NCBIMGSCv37MGSCv37mm9NCBIm37
MGSCv36X136,011,320 - 136,114,080 (+)NCBIMGSCv36mm8
CeleraX123,937,263 - 124,027,053 (+)NCBICelera
Cytogenetic MapXF1NCBI
cM MapX61.35NCBI
Mid2
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0 EnsemblNW_0049554907,973,211 - 8,088,427 (-)Ensembl
ChiLan1.0NW_0049554907,977,771 - 8,168,912 (-)NCBIChiLan1.0ChiLan1.0
MID2
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
NHGRI_mPanPan1-v2X107,301,285 - 107,405,554 (+)NCBINHGRI_mPanPan1-v2
NHGRI_mPanPan1X107,304,702 - 107,413,484 (+)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v0X96,917,731 - 97,026,545 (+)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.1X107,290,776 - 107,393,162 (+)NCBIPanPan1.1PanPan1.1panPan2
PanPan1.1 EnsemblX107,290,776 - 107,393,162 (+)EnsemblpanPan2panpan1.1
MID2
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
CanFam3.1X81,267,037 - 81,467,613 (+)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 EnsemblX81,296,249 - 81,463,210 (+)EnsemblcanFam3CanFam3.1
Dog10K_Boxer_TashaX67,387,281 - 67,588,271 (+)NCBIDog10K_Boxer_TashaDog10K_Boxer_Tasha
ROS_Cfam_1.0X82,914,364 - 83,116,618 (+)NCBIROS_Cfam_1.0ROS_Cfam_1.0
ROS_Cfam_1.0 EnsemblX82,942,999 - 83,112,209 (+)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.1X80,374,592 - 80,575,813 (+)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.0X82,086,451 - 82,288,627 (+)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.0X81,878,438 - 82,081,198 (+)NCBIUU_Cfam_GSD_1.0UU_Cfam_GSD_1.0
Mid2
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2X80,731,399 - 80,828,234 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_0049364996,829,729 - 6,926,571 (-)EnsemblSpeTri2.0 Ensembl
SpeTri2.0NW_0049364996,833,328 - 6,926,531 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
MID2
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1 EnsemblX88,294,067 - 88,393,742 (+)EnsemblsusScr11Sscrofa11.1
Sscrofa11.1X88,294,032 - 88,394,885 (+)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2X102,730,773 - 102,831,117 (+)NCBISscrofa10.2Sscrofa10.2susScr3
MID2
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChlSab1.1X95,664,705 - 95,760,256 (+)NCBIChlSab1.1ChlSab1.1chlSab2
ChlSab1.1 EnsemblX95,665,380 - 95,764,762 (+)EnsemblChlSab1.1 EnsemblchlSab2Vervet-AGM
Vero_WHO_p1.0NW_02366606520,771,575 - 20,871,556 (+)NCBIVero_WHO_p1.0Vero_WHO_p1.0
Mid2
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_0046248038,536,953 - 8,650,805 (-)EnsemblHetGla_female_1.0 EnsemblhetGla2HetGla_female_1.0 Ensembl
HetGla 1.0NW_0046248038,536,946 - 8,650,775 (-)NCBIHetGla 1.0HetGla 1.0hetGla2
Mid2
(Rattus rattus - black rat)
Black Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Rrattus_CSIRO_v1X93,082,103 - 93,179,939 (+)NCBIRrattus_CSIRO_v1

Variants

.
Variants in Mid2
476 total Variants
miRNA Target Status (No longer updated)

Predicted Target Of
Summary Value
Count of predictions:35
Count of miRNA genes:29
Interacting mature miRNAs:33
Transcripts:ENSRNOT00000020145
Prediction methods:Miranda, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (GRCr8)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1598872Memor14Memory QTL 144.5exploratory behavior trait (VT:0010471)difference between duration of physical contact or close proximity of test subject and social stimulus during sample phase and test phase (CMO:0002678)X97528096142528096Rat
738025Stresp3Stress response QTL 34.610.0066stress-related behavior trait (VT:0010451)defensive burying - approachX105359969157758123Rat
61430Cia18Collagen induced arthritis QTL 183.1joint integrity trait (VT:0010548)joint inflammation composite score (CMO:0000919)X80434318125434318Rat
738029Stresp2Stress response QTL 23.40.0004stress-related behavior trait (VT:0010451)defensive burying - approachX92979612117740458Rat
61431Cia19Collagen induced arthritis QTL 194.4joint integrity trait (VT:0010548)joint inflammation composite score (CMO:0000919)X105468858125434318Rat
738035Stresp1Stress response QTL 14.960.000011stress-related behavior trait (VT:0010451)defensive burying - copingX45182909117740458Rat
2302047Pia34Pristane induced arthritis QTL 345.80.001blood immunoglobulin amount (VT:0002460)serum immunoglobulin G1 level (CMO:0002115)1996916163141916163Rat


Expression

RNA-SEQ Expression

alimentary part of gastrointestinal system
appendage
circulatory system
ectoderm
endocrine system
endoderm
exocrine system
hemolymphoid system
hepatobiliary system
integumental system
mesenchyme
mesoderm
musculoskeletal system
nervous system
renal system
reproductive system
respiratory system
sensory system
visual system
16 12 67 161 91 90 59 88 59 6 352 188 11 140 81 92 31 16 16

Sequence


Ensembl Acc Id: ENSRNOT00000079841   ⟹   ENSRNOP00000069903
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 EnsemblX109,143,820 - 109,241,973 (+)Ensembl
mRatBN7.2 EnsemblX104,355,316 - 104,453,473 (+)Ensembl
Rnor_6.0 EnsemblX112,020,646 - 112,121,943 (+)Ensembl
Ensembl Acc Id: ENSRNOT00000118902   ⟹   ENSRNOP00000101518
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 EnsemblX109,143,253 - 109,245,255 (+)Ensembl
Ensembl Acc Id: ENSRNOT00000143485   ⟹   ENSRNOP00000107382
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 EnsemblX109,143,820 - 109,241,973 (+)Ensembl
RefSeq Acc Id: NM_001191889   ⟹   NP_001178818
RefSeq Status: VALIDATED
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8X109,143,820 - 109,241,973 (+)NCBI
mRatBN7.2X104,355,316 - 104,453,473 (+)NCBI
Rnor_6.0X112,020,565 - 112,118,696 (+)NCBI
Rnor_5.0352,479,569 - 52,581,663 (-)NCBI
Celera343,484,990 - 43,583,167 (-)NCBI
Sequence:
RefSeq Acc Id: XM_006234247   ⟹   XP_006234309
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8X109,143,416 - 109,245,257 (+)NCBI
mRatBN7.2X104,354,900 - 104,456,757 (+)NCBI
Rnor_6.0X112,020,142 - 112,121,980 (+)NCBI
Rnor_5.0352,479,569 - 52,581,663 (-)NCBI
Sequence:
RefSeq Acc Id: XM_006234248   ⟹   XP_006234310
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8X109,143,173 - 109,245,257 (+)NCBI
mRatBN7.2X104,354,692 - 104,456,757 (+)NCBI
Rnor_6.0X112,019,897 - 112,121,980 (+)NCBI
Rnor_5.0352,479,569 - 52,581,663 (-)NCBI
Sequence:
RefSeq Acc Id: XM_063280153   ⟹   XP_063136223
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8X109,143,057 - 109,245,257 (+)NCBI
RefSeq Acc Id: NP_001178818   ⟸   NM_001191889
- UniProtKB: A0A0G2JWJ3 (UniProtKB/TrEMBL)
- Sequence:
RefSeq Acc Id: XP_006234310   ⟸   XM_006234248
- Peptide Label: isoform X3
- Sequence:
RefSeq Acc Id: XP_006234309   ⟸   XM_006234247
- Peptide Label: isoform X1
- Sequence:
Ensembl Acc Id: ENSRNOP00000069903   ⟸   ENSRNOT00000079841
RefSeq Acc Id: XP_063136223   ⟸   XM_063280153
- Peptide Label: isoform X2
Ensembl Acc Id: ENSRNOP00000107382   ⟸   ENSRNOT00000143485
Ensembl Acc Id: ENSRNOP00000101518   ⟸   ENSRNOT00000118902
Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-A0A0G2JWJ3-F1-model_v2 AlphaFold A0A0G2JWJ3 1-705 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen


Additional Information

Database Acc Id Source(s)
AGR Gene RGD:1565674 AgrOrtholog
BioCyc Gene G2FUF-1540 BioCyc
Ensembl Genes ENSRNOG00000060542 Ensembl, ENTREZGENE, UniProtKB/TrEMBL
Ensembl Transcript ENSRNOT00000079841 ENTREZGENE
  ENSRNOT00000079841.3 UniProtKB/TrEMBL
  ENSRNOT00000118902 ENTREZGENE
  ENSRNOT00000118902.2 UniProtKB/TrEMBL
  ENSRNOT00000143485.1 UniProtKB/TrEMBL
Gene3D-CATH 2.60.120.920 UniProtKB/TrEMBL
  2.60.40.10 UniProtKB/TrEMBL
  3.30.40.10 UniProtKB/TrEMBL
  4.10.830.40 UniProtKB/TrEMBL
  Classic Zinc Finger UniProtKB/TrEMBL
InterPro B30.2/SPRY_sf UniProtKB/TrEMBL
  B302 UniProtKB/TrEMBL
  Bbox_C UniProtKB/TrEMBL
  Butyrophylin UniProtKB/TrEMBL
  ConA-like_dom UniProtKB/TrEMBL
  COS_domain UniProtKB/TrEMBL
  E3_ligase_FN3/SPRY UniProtKB/TrEMBL
  FN3_sf UniProtKB/TrEMBL
  FN_III UniProtKB/TrEMBL
  Ig-like_fold UniProtKB/TrEMBL
  MID2 UniProtKB/TrEMBL
  MID2_Bbox1_Zfn UniProtKB/TrEMBL
  MID2_Bbox2_Zfn UniProtKB/TrEMBL
  Midline-1_COS UniProtKB/TrEMBL
  SPRY/PRY_TRIM1 UniProtKB/TrEMBL
  SPRY_rcpt UniProtKB/TrEMBL
  Znf-RING_LisH UniProtKB/TrEMBL
  Znf_B-box UniProtKB/TrEMBL
  Znf_RING UniProtKB/TrEMBL
  Znf_RING/FYVE/PHD UniProtKB/TrEMBL
  Znf_RING_CS UniProtKB/TrEMBL
KEGG Report rno:363502 UniProtKB/TrEMBL
NCBI Gene 363502 ENTREZGENE
PANTHER E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED UniProtKB/TrEMBL
  PTHR24099:SF12 UniProtKB/TrEMBL
Pfam ANCHR-like_BBOX UniProtKB/TrEMBL
  COS UniProtKB/TrEMBL
  fn3 UniProtKB/TrEMBL
  SPRY UniProtKB/TrEMBL
  zf-B_box UniProtKB/TrEMBL
  zf-RING_UBOX UniProtKB/TrEMBL
PhenoGen Mid2 PhenoGen
PRINTS BUTYPHLNCDUF UniProtKB/TrEMBL
PROSITE B302_SPRY UniProtKB/TrEMBL
  COS UniProtKB/TrEMBL
  FN3 UniProtKB/TrEMBL
  ZF_BBOX UniProtKB/TrEMBL
  ZF_RING_1 UniProtKB/TrEMBL
  ZF_RING_2 UniProtKB/TrEMBL
RatGTEx ENSRNOG00000060542 RatGTEx
SMART BBC UniProtKB/TrEMBL
  BBOX UniProtKB/TrEMBL
  FN3 UniProtKB/TrEMBL
  RING UniProtKB/TrEMBL
  SPRY UniProtKB/TrEMBL
Superfamily-SCOP B-box zinc-binding domain UniProtKB/TrEMBL
  ConA_like_lec_gl UniProtKB/TrEMBL
  FN_III-like UniProtKB/TrEMBL
  RING/U-box UniProtKB/TrEMBL
UniProt A0A0G2JWJ3 ENTREZGENE, UniProtKB/TrEMBL
  A0ABK0LKT0_RAT UniProtKB/TrEMBL
  A0ABK0LNP2_RAT UniProtKB/TrEMBL
  A6HLR4 ENTREZGENE, UniProtKB/TrEMBL


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2008-04-30 Mid2  midline 2   Mid2_predicted  midline 2 (predicted)  'predicted' is removed 2292626 APPROVED
2006-03-30 Mid2_predicted  midline 2 (predicted)  RGD1565674_predicted  similar to Trim1 alpha (predicted)  Symbol and Name updated 1299863 APPROVED
2006-03-07 RGD1565674_predicted  similar to Trim1 alpha (predicted)  LOC363502  similar to Trim1 alpha  Symbol and Name status set to approved 1299863 APPROVED
2006-02-09 LOC363502  similar to Trim1 alpha      Symbol and Name status set to provisional 70820 PROVISIONAL