ENCODES a protein that exhibits beta-catenin binding (ortholog); chromatin binding (ortholog); cis-regulatory region sequence-specific DNA binding (ortholog); INVOLVED IN adrenal gland development (ortholog); branching involved in ureteric bud morphogenesis (ortholog); embryonic digestive tract deve
Sall1 mutation was induced by injecting a mix of two pX330 expressing Cas9 and sgRNA targeting the sequence into Crlj:WI rat embryos. The resulting mutation is a 4456-bp deletion in exon 2 to 3. Homozygous Sall1 knocked-out
Targeting vector was designed to replace 2nd and 3rd exons encoding DNA-binding domain of Sall1 locus with tdTomato. The vector was introduced into WDB/Nips-ES1/Nips (RGD ID:10054010) embryonic stem cells by electroporation. Founder animals were backcrossed to C