BAC clone NC1-269B17 which containing about 120 kb of ACI/NJcl rat-derived Casp3 (caspase 3) was injected into F344/Jcl embryos. The founder rat was backcrossed into F344/Jcl rat to establish the transgenic strain.
This transgenic rat possesses three copies of BAC clone. Hemizygous rat expresses higher level of Casp3 protein in adenocarcinoma of the large intestine than that of wild type rat. In crossing a male Tg rat with female F344/Jcl, males are all non-Tg and females are all Tg, suggesting that Tg was introduced onto chromosome X