Male founders are injected with ENU (N-ethyl-N-nitrosourea) and harem bred to females. The pups are genetically screened using the TILLING assay (an enzyme-based heteroduplex cleavage assay) as well as nucleotide sequencing to identify and characterize target genes possessing ENU-induced mutations. E124D mutation is generated from the codon change GAG/GAT.
Last Known Status:
Live Animals; Cryopreserved Sperm (as of 2017-01-26)