RGD Reference Report - Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis. - Rat Genome Database

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Secretory leukocyte protease inhibitor is an inducible antimicrobial peptide expressed in Staphylococcus aureus endophthalmitis.

Authors: Reviglio, VE  Sambuelli, RH  Olmedo, A  Falco, M  Echenique, J  O'Brien, TP  Kuo, IC 
Citation: Reviglio VE, etal., Mediators Inflamm. 2007;2007:93857. doi: 10.1155/2007/93857.
RGD ID: 9999421
Pubmed: PMID:18274645   (View Abstract at PubMed)
PMCID: PMC2234354   (View Article at PubMed Central)
DOI: DOI:10.1155/2007/93857   (Journal Full-text)

PURPOSE: To describe the presence of secretory leukocyte protease inhibitor (SLPI), a cationic peptide with antimicrobial and antiprotease activity, in the innate ocular immune reaction in a rat model of Staphylococcus aureus endophthalmitis. METHODS: Seventy-five female Lewis rats were divided into three groups: the endophthalmitis group received an intravitreal injection of 65 colony-forming units of viable S. aureus, the vehicle-injected group received balanced sterile saline solution (BSS), and the control group was not injected. Eyes were enucleated at 24 and 48 hours and processed for immunohistochemical staining and Western blot studies for SLPI. RESULTS: In S. aureus endophthalmitis eyes, there was strong immunostaining for SLPI in the retina and vitreous with associated neutrophilic infiltrates. At 48 hours, corneas also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. By immunohistochemical assays, SLPI was absent in the BSS and control eyes. The causative pathogen was identified in all samples from the endophthalmitis group by traditional culture methods. CONCLUSIONS: To our knowledge, this report is the first to demonstrate the presence of SLPI in the inflamed cornea, vitreous, and retina tissues of rat eyes with S. aureus endophthalmitis, suggesting that SLPI has an active role in the innate immunity of the eye. Release of SLPI by inflammatory cells in the anterior and posterior segments may contribute to the host defense response against infectious endophthalmitis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endophthalmitis  ISOSlpi (Rattus norvegicus)9999421; 9999421associated with Staphylococcal Infections more ...RGD 
endophthalmitis  IEP 9999421associated with Staphylococcal Infections more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slpi  (secretory leukocyte peptidase inhibitor)

Genes (Mus musculus)
Slpi  (secretory leukocyte peptidase inhibitor)

Genes (Homo sapiens)
SLPI  (secretory leukocyte peptidase inhibitor)


Additional Information