RGD Reference Report - Expression of smooth muscle myosin heavy chain B in cardiac vessels of normotensive and hypertensive rats. - Rat Genome Database

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Expression of smooth muscle myosin heavy chain B in cardiac vessels of normotensive and hypertensive rats.

Authors: Wetzel, U  Lutsch, G  Haase, H  Ganten, U  Morano, I 
Citation: Wetzel U, etal., Circ Res. 1998 Jul 27;83(2):204-9.
RGD ID: 9835388
Pubmed: PMID:9686760   (View Abstract at PubMed)

We investigated expression of the 5'-spliced isoform of smooth muscle myosin heavy chain (SM-MHC-B) in smooth muscle cells of cardiac vessels of the left ventricle of normotensive (Wistar-Kyoto) and spontaneously hypertensive rats of the stroke-prone strain by immunofluorescence microscopy. In parallel, liver and bladder were studied for characterization of the nature of vessels expressing SM-MHC-B and for semiquantitative evaluation of its abundance. Smooth muscle cells were detected by staining with a monoclonal antibody specific for alpha-smooth muscle actin. Abundance of the SM-MHC-B isoform in these cells was evaluated by using an antibody raised against the seven-amino acid insert at the 25K/50K junction of the myosin head (a25K/50K) that specifically recognized SM-MHC-B. In the ventricle, a25K/50K immunoreactivity was observed in smooth muscle cells of precapillary arterioles but not in larger vessels or aorta. The a25K/50K immunoresponse of those vessels with the highest expression level of SM-MHC-B closely resembled the signal observed in the smooth muscle layer of urinary bladder known to preferentially express SM-MHC-B. Interestingly, in left ventricles of stroke-prone spontaneously hypertensive rats, there was a significantly reduced fraction of a25K/50K-positive precapillary arterioles compared with normotensive control rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypertension  ISOMyh7 (Rattus norvegicus)9835388; 9835388protein:decreased expression:heart left ventricle and arterioleRGD 
hypertension  IEP 9835388protein:decreased expression:heart left ventricle and arterioleRGD 

Objects Annotated

Genes (Rattus norvegicus)
Myh7  (myosin heavy chain 7)

Genes (Mus musculus)
Myh7  (myosin, heavy polypeptide 7, cardiac muscle, beta)

Genes (Homo sapiens)
MYH7  (myosin heavy chain 7)


Additional Information