RGD Reference Report - Secreted nucleobindin-2 inhibits 3T3-L1 adipocyte differentiation. - Rat Genome Database

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Secreted nucleobindin-2 inhibits 3T3-L1 adipocyte differentiation.

Authors: Tagaya, Y  Osaki, A  Miura, A  Okada, S  Ohshima, K  Hashimoto, K  Yamada, M  Satoh, T  Shimizu, H  Mori, M 
Citation: Tagaya Y, etal., Protein Pept Lett. 2012 Sep;19(9):997-1004.
RGD ID: 9831186
Pubmed: PMID:22486620   (View Abstract at PubMed)
PMCID: PMC3468916   (View Article at PubMed Central)

Nucleobindin-2 is a 420 amino acid EF-hand Ca(2)(+) binding protein that can be further processed to generate an 82 amino terminal peptide termed Nesfatin-1. To examine the function of secreted Nucleobindin-2 in adipocyte differentiation, cultured 3T3-L1 cells were incubated with either 0 or 100 nM of GST, GST-Nucleobindin-2, prior to and during the initiation of adipocyte differentiation. Nucleobindin-2 treatment decreased neutral lipid accumulation (Oil-Red O staining) and expression of several marker genes for adipocyte differentiation (PPARgamma, aP2, and adipsin). When Nucleobindin- 2 was constitutively secreted into cultured medium, cAMP content and insulin stimulated CREB phosphorylation were significantly reduced. On the other hand, intracellularly overexpressed Nucleobindin-2 failed to affect cAMP content and CREB phosphorylation. Taken together, these data indicate that secreted Nucleobindin-2 is a suppressor of adipocyte differentiation through inhibition of cAMP production and insulin signal.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Nucb2  (nucleobindin 2)


Additional Information