RGD Reference Report - Voltage-gated K+ channel beta subunits: expression and distribution of Kv beta 1 and Kv beta 2 in adult rat brain. - Rat Genome Database

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Voltage-gated K+ channel beta subunits: expression and distribution of Kv beta 1 and Kv beta 2 in adult rat brain.

Authors: Rhodes, KJ  Monaghan, MM  Barrezueta, NX  Nawoschik, S  Bekele-Arcuri, Z  Matos, MF  Nakahira, K  Schechter, LE  Trimmer, JS 
Citation: Rhodes KJ, etal., J Neurosci. 1996 Aug 15;16(16):4846-60.
RGD ID: 9743934
Pubmed: PMID:8756417   (View Abstract at PubMed)
PMCID: PMC6579302   (View Article at PubMed Central)

Recent cloning of K+ channel beta subunits revealed that these cytoplasmic polypeptides can dramatically alter the kinetics of current inactivation and promote efficient glycosylation and surface expression of the channel-forming alpha subunits. Here, we examined the expression, distribution, and association of two of these beta subunits, Kv beta 1 and Kv beta 2, in adult rat brain. In situ hybridization using cRNA probes revealed that these beta-subunit genes are heterogeneously expressed, with high densities of Kv beta 1 mRNA in the striatum, CA1 subfield of the hippocampus, and cerebellar Purkinje cells, and high densities of Kv beta 2 mRNA in the cerebral cortex, cerebellum, and brainstem. Immunohistochemical staining using subunit-specific monoclonal and affinity-purified polyclonal antibodies revealed that the Kv beta 1 and Kv beta 2 polypeptides frequently co-localize and are concentrated in neuronal perikarya, dendrites, and terminal fields, and in the juxtaparanodal region of myelinated axons. Immunoblot and reciprocal co-immunoprecipitation analyses indicated that Kv beta 2 is the major beta subunit present in rat brain membranes, and that most K+ channel complexes containing Kv beta 1 also contain Kv beta 2. Taken together, these data suggest that Kv beta 2 is a component of almost all K+ channel complexes containing Kv 1 alpha subunits, and that individual channels may contain two or more biochemically and functionally distinct beta-subunit polypeptides.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon  IDA 9743934 RGD 
dendrite cytoplasm  IDA 9743934 RGD 
juxtaparanode region of axon  IDA 9743934 RGD 
neuronal cell body  IDA 9743934 RGD 
perikaryon  IDA 9743934 RGD 
proximal dendrite  IDA 9743934 RGD 
voltage-gated potassium channel complex  IDA 9743934 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Kcnab1  (potassium voltage-gated channel subfamily A regulatory beta subunit 1)
Kcnab2  (potassium voltage-gated channel subfamily A regulatory beta subunit 2)

Objects referenced in this article
Gene Kcnab1 potassium voltage-gated channel, shaker-related subfamily, beta member 1 Mus musculus

Additional Information