RGD Reference Report - Overexpression of Eag1 potassium channels in clinical tumours. - Rat Genome Database

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Overexpression of Eag1 potassium channels in clinical tumours.

Authors: Hemmerlein, B  Weseloh, RM  Mello de Queiroz, F  Knotgen, H  Sanchez, A  Rubio, ME  Martin, S  Schliephacke, T  Jenke, M  Heinz-Joachim-Radzun, M  Stuhmer, W  Pardo, LA 
Citation: Hemmerlein B, etal., Mol Cancer. 2006 Oct 5;5:41.
RGD ID: 9693723
Pubmed: PMID:17022810   (View Abstract at PubMed)
PMCID: PMC1621079   (View Article at PubMed Central)
DOI: DOI:10.1186/1476-4598-5-41   (Journal Full-text)

BACKGROUND: Certain types of potassium channels (known as Eag1, KCNH1, Kv10.1) are associated with the production of tumours in patients and in animals. We have now studied the expression pattern of the Eag1 channel in a large range of normal and tumour tissues from different collections utilising molecular biological and immunohistochemical techniques. RESULTS: The use of reverse transcription real-time PCR and specifically generated monoclonal anti-Eag1 antibodies showed that expression of the channel is normally limited to specific areas of the brain and to restricted cell populations throughout the body. Tumour samples, however, showed a significant overexpression of the channel with high frequency (up to 80% depending on the tissue source) regardless of the detection method (staining with either one of the antibodies, or detection of Eag1 RNA). CONCLUSION: Inhibition of Eag1 expression in tumour cell lines reduced cell proliferation. Eag1 may therefore represent a promising target for the tailored treatment of human tumours. Furthermore, as normal cells expressing Eag1 are either protected by the blood-brain barrier or represent the terminal stage of normal differentiation, Eag1 based therapies could produce only minor side effects.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hepatocellular carcinoma  IEP 9693723protein:increased expression:liver (human)RGD 
hepatocellular carcinoma  ISOKCNH1 (Homo sapiens)9693723; 9693723protein:increased expression:liver (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnh1  (potassium voltage-gated channel subfamily H member 1)

Genes (Mus musculus)
Kcnh1  (potassium voltage-gated channel, subfamily H (eag-related), member 1)

Genes (Homo sapiens)
KCNH1  (potassium voltage-gated channel subfamily H member 1)


Additional Information