RGD Reference Report - Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-a-go-go channels in a stably transfected cell line. - Rat Genome Database

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Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-a-go-go channels in a stably transfected cell line.

Authors: Stansfeld, CE  Roper, J  Ludwig, J  Weseloh, RM  Marsh, SJ  Brown, DA  Pongs, O 
Citation: Stansfeld CE, etal., Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9910-4.
RGD ID: 9693720
Pubmed: PMID:8790430   (View Abstract at PubMed)
PMCID: PMC38528   (View Article at PubMed Central)

We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of calcium-dependent kinases and phosphatases. The effect of calcium on r-eag channel activity was studied in inside-out membrane patches. Calcium inhibited r-eag channel activity with a mean IC50 of 67 nM. Activation of muscarinic receptors, generating calcium oscillations in the transfected cells, induced a synchronous inhibition of r-eag mediated outward currents. This shows that calcium can mediate r-eag current inhibition following muscarinic receptor activation. The data indicate that r-eag channels are calcium-inhibitable voltage-activated potassium channels.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to calcium ion  IDA 9693720 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnh1  (potassium voltage-gated channel subfamily H member 1)


Additional Information