RGD Reference Report - Enhancement of dorsal hippocampal activity by knockdown of HCN1 channels leads to anxiolytic- and antidepressant-like behaviors. - Rat Genome Database

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Enhancement of dorsal hippocampal activity by knockdown of HCN1 channels leads to anxiolytic- and antidepressant-like behaviors.

Authors: Kim, CS  Chang, PY  Johnston, D 
Citation: Kim CS, etal., Neuron. 2012 Aug 9;75(3):503-16. doi: 10.1016/j.neuron.2012.05.027.
RGD ID: 9686419
Pubmed: PMID:22884333   (View Abstract at PubMed)
PMCID: PMC3418514   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neuron.2012.05.027   (Journal Full-text)

The hippocampus is an integral brain region for affective disorders. TRIP8b knockout mice lacking functional HCN channels as well as both HCN1 and HCN2 knockout mice have been shown to display antidepressant-like behaviors. The mechanisms or brain regions involved in these alterations in behavior, however, are not clear. We developed a lentiviral shRNA system to examine whether knockdown of HCN1 protein in the dorsal hippocampal CA1 region is sufficient to produce antidepressant-like effects. We found that knockdown of HCN1 channels increased cellular excitability and resulted in physiological changes consistent with a reduction of I(h). Rats infused with lentiviral shRNA-HCN1 in the dorsal hippocampal CA1 region displayed antidepressant- and anxiolytic-like behaviors associated with widespread enhancement of hippocampal activity and upregulation of BDNF-mTOR signaling pathways. Our results suggest that HCN1 protein could be a potential target for treatment of anxiety and depression disorders.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
anxiety disorder  ISOHcn1 (Rattus norvegicus)9686419; 9686419 RGD 
anxiety disorder  IMP 9686419 RGD 
mood disorder  ISOHcn1 (Rattus norvegicus)9686419; 9686419 RGD 
mood disorder  IMP 9686419 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of action potential  IMP 9686419 RGD 
regulation of membrane hyperpolarization  IMP 9686419 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hcn1  (hyperpolarization-activated cyclic nucleotide-gated potassium channel 1)

Genes (Mus musculus)
Hcn1  (hyperpolarization activated cyclic nucleotide gated potassium channel 1)

Genes (Homo sapiens)
HCN1  (hyperpolarization activated cyclic nucleotide gated potassium channel 1)


Additional Information