RGD Reference Report - Differential expression of HCN subunits alters voltage-dependent gating of h-channels in CA1 pyramidal neurons from dorsal and ventral hippocampus. - Rat Genome Database

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Differential expression of HCN subunits alters voltage-dependent gating of h-channels in CA1 pyramidal neurons from dorsal and ventral hippocampus.

Authors: Dougherty, KA  Nicholson, DA  Diaz, L  Buss, EW  Neuman, KM  Chetkovich, DM  Johnston, D 
Citation: Dougherty KA, etal., J Neurophysiol. 2013 Apr;109(7):1940-53. doi: 10.1152/jn.00010.2013. Epub 2013 Jan 16.
RGD ID: 9686375
Pubmed: PMID:23324324   (View Abstract at PubMed)
PMCID: PMC3628004   (View Article at PubMed Central)
DOI: DOI:10.1152/jn.00010.2013   (Journal Full-text)

The rodent hippocampus can be divided into dorsal (DHC) and ventral (VHC) domains on the basis of behavioral, anatomical, and biochemical differences. Recently, we reported that CA1 pyramidal neurons from the VHC were intrinsically more excitable than DHC neurons, but the specific ionic conductances contributing to this difference were not determined. Here we investigated the hyperpolarization-activated current (I(h)) and the expression of HCN1 and HCN2 channel subunits in CA1 pyramidal neurons from the DHC and VHC. Measurement of Ih with cell-attached patches revealed a significant depolarizing shift in the V(1/2) of activation for dendritic h-channels in VHC neurons (but not DHC neurons), and ultrastructural immunolocalization of HCN1 and HCN2 channels revealed a significantly larger HCN1-to-HCN2 ratio for VHC neurons (but not DHC neurons). These observations suggest that a shift in the expression of HCN1 and HCN2 channels drives functional changes in I(h) for VHC neurons (but not DHC neurons) and could thereby significantly alter the capacity for dendritic integration of these neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendrite membrane  IDA 9686375; 9686375 RGD 
somatodendritic compartment  IDA 9686375; 9686375 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hcn1  (hyperpolarization-activated cyclic nucleotide-gated potassium channel 1)
Hcn2  (hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2)


Additional Information