RGD Reference Report - Kv3.1-Kv3.2 channels underlie a high-voltage-activating component of the delayed rectifier K+ current in projecting neurons from the globus pallidus. - Rat Genome Database

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Kv3.1-Kv3.2 channels underlie a high-voltage-activating component of the delayed rectifier K+ current in projecting neurons from the globus pallidus.

Authors: Hernandez-Pineda, R  Chow, A  Amarillo, Y  Moreno, H  Saganich, M  Vega-Saenz de Miera, EC  Hernandez-Cruz, A  Rudy, B 
Citation: Hernandez-Pineda R, etal., J Neurophysiol. 1999 Sep;82(3):1512-28.
RGD ID: 9685780
Pubmed: PMID:10482766   (View Abstract at PubMed)

The globus pallidus plays central roles in the basal ganglia circuitry involved in movement control as well as in cognitive and emotional functions. There is therefore great interest in the anatomic and electrophysiological characterization of this nucleus. Most pallidal neurons are GABAergic projecting cells, a large fraction of which express the calcium binding protein parvalbumin (PV). Here we show that PV-containing pallidal neurons coexpress Kv3. 1 and Kv3.2 K+ channel proteins and that both Kv3.1 and Kv3.2 antibodies coprecipitate both channel proteins from pallidal membrane extracts solubilized with nondenaturing detergents, suggesting that the two channel subunits are forming heteromeric channels. Kv3.1 and Kv3.2 channels have several unusual electrophysiological properties when expressed in heterologous expression systems and are thought to play special roles in neuronal excitability including facilitating sustained high-frequency firing in fast-spiking neurons such as interneurons in the cortex and the hippocampus. Electrophysiological analysis of freshly dissociated pallidal neurons demonstrates that these cells have a current that is nearly identical to the currents expressed by Kv3.1 and Kv3.2 proteins in heterologous expression systems, including activation at very depolarized membrane potentials (more positive than -10 mV) and very fast deactivation rates. These results suggest that the electrophysiological properties of native channels containing Kv3.1 and Kv3.2 proteins in pallidal neurons are not significantly affected by factors such as associated subunits or postranslational modifications that result in channels having different properties in heterologous expression systems and native neurons. Most neurons in the globus pallidus have been reported to fire sustained trains of action potentials at high-frequency. Kv3.1-Kv3.2 voltage-gated K+ channels may play a role in helping maintain sustained high-frequency repetitive firing as they probably do in other neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to ammonium ion  IEP 9685780tetraethylammoniumRGD 
globus pallidus development  IEP 9685780; 9685780 RGD 
potassium ion transmembrane transport involved_inIDA 9685780PMID:10482766UniProt 
protein heterooligomerization involved_inIDA 9685780PMID:10482766UniProt 
protein homooligomerization involved_inIDA 9685780PMID:10482766UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuronal cell body membrane located_inIDA 9685780PMID:10482766UniProt 
perikaryon located_inIDA 9685780PMID:10482766UniProt 
voltage-gated potassium channel complex part_ofIDA 9685780PMID:10482766UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Kcnc1  (potassium voltage-gated channel subfamily C member 1)
Kcnc2  (potassium voltage-gated channel subfamily C member 2)


Additional Information