RGD Reference Report - Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. - Rat Genome Database

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Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.

Authors: Hu, P  Wu, S  Sun, Y  Yuan, CC  Kobayashi, R  Myers, MP  Hernandez, N 
Citation: Hu P, etal., Mol Cell Biol. 2002 Nov;22(22):8044-55.
RGD ID: 9685217
Pubmed: PMID:12391170   (View Abstract at PubMed)
PMCID: PMC134740   (View Article at PubMed Central)

Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of similarity to the yeast subunits. We have purified an RNA polymerase III complex and identified its components. We found that two RNA polymerase III subunits, referred to as RPC8 and RPC9, displayed sequence similarity to the RNA polymerase II RPB7 and RPB4 subunits, respectively. RPC8 and RPC9 associated with each other, paralleling the association of the RNA polymerase II subunits, and were thus paralogues of RPB7 and RPB4. Furthermore, the complex contained a prominent 80-kDa polypeptide, which we called RPC5 and which corresponded to the human orthologue of the yeast C37 subunit despite limited sequence similarity. RPC5 associated with RPC53, the human orthologue of S. cerevisiae C53, paralleling the association of the S. cerevisiae C37 and C53 subunits, and was required for transcription from the type 2 VAI and type 3 human U6 promoters. Our results provide a characterization of human RNA polymerase III and show that the RPC5 subunit is essential for transcription.

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
RNA polymerase III transcription pathway   TAS 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOCRCP (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR1C (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR1D (Homo sapiens)9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR2E (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR2F (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR2H (Homo sapiens)9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR2K (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR2L (Homo sapiens)9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3A (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3B (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3C (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3D (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3E (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3F (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3G (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3H (Homo sapiens)9685217; 9685217 RGD 
RNA polymerase III transcription pathway   ISOPOLR3K (Homo sapiens)9685217; 9685217 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Crcp  (CGRP receptor component)
Polr1c  (RNA polymerase I and III subunit C)
Polr2e  (RNA polymerase II, I and III subunit E)
Polr2f  (RNA polymerase II, I and III subunit F)
Polr2k  (RNA polymerase II, I and III subunit K)
Polr3a  (RNA polymerase III subunit A)
Polr3b  (RNA polymerase III subunit B)
Polr3c  (RNA polymerase III subunit C)
Polr3d  (RNA polymerase III subunit D)
Polr3e  (RNA polymerase III subunit E)
Polr3f  (RNA polymerase III subunit F)
Polr3g  (RNA polymerase III subunit G)
Polr3h  (RNA polymerase III subunit H)
Polr3k  (RNA polymerase III subunit K)

Genes (Mus musculus)
Crcp  (calcitonin gene-related peptide-receptor component protein)
Polr1c  (polymerase (RNA) I polypeptide C)
Polr1d  (polymerase (RNA) I polypeptide D)
Polr2e  (polymerase (RNA) II (DNA directed) polypeptide E)
Polr2f  (polymerase (RNA) II (DNA directed) polypeptide F)
Polr2h  (polymerase (RNA) II (DNA directed) polypeptide H)
Polr2k  (polymerase (RNA) II (DNA directed) polypeptide K)
Polr2l  (polymerase (RNA) II (DNA directed) polypeptide L)
Polr3a  (polymerase (RNA) III (DNA directed) polypeptide A)
Polr3b  (polymerase (RNA) III (DNA directed) polypeptide B)
Polr3c  (polymerase (RNA) III (DNA directed) polypeptide C)
Polr3d  (polymerase (RNA) III (DNA directed) polypeptide D)
Polr3e  (polymerase (RNA) III (DNA directed) polypeptide E)
Polr3f  (polymerase (RNA) III (DNA directed) polypeptide F)
Polr3g  (polymerase (RNA) III (DNA directed) polypeptide G)
Polr3h  (polymerase (RNA) III (DNA directed) polypeptide H)
Polr3k  (polymerase (RNA) III (DNA directed) polypeptide K)

Genes (Homo sapiens)
CRCP  (CGRP receptor component)
POLR1C  (RNA polymerase I and III subunit C)
POLR1D  (RNA polymerase I and III subunit D)
POLR2E  (RNA polymerase II, I and III subunit E)
POLR2F  (RNA polymerase II, I and III subunit F)
POLR2H  (RNA polymerase II, I and III subunit H)
POLR2K  (RNA polymerase II, I and III subunit K)
POLR2L  (RNA polymerase II, I and III subunit L)
POLR3A  (RNA polymerase III subunit A)
POLR3B  (RNA polymerase III subunit B)
POLR3C  (RNA polymerase III subunit C)
POLR3D  (RNA polymerase III subunit D)
POLR3E  (RNA polymerase III subunit E)
POLR3F  (RNA polymerase III subunit F)
POLR3G  (RNA polymerase III subunit G)
POLR3H  (RNA polymerase III subunit H)
POLR3K  (RNA polymerase III subunit K)


Additional Information