RGD Reference Report - Hepatic calcium-binding protein regucalcin concentration is decreased by streptozotocin-diabetic state and ethanol ingestion in rats. - Rat Genome Database

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Hepatic calcium-binding protein regucalcin concentration is decreased by streptozotocin-diabetic state and ethanol ingestion in rats.

Authors: Isogai, M  Kurota, H  Yamaguchi, M 
Citation: Isogai M, etal., Mol Cell Biochem. 1997 Mar;168(1-2):67-72.
RGD ID: 9590177
Pubmed: PMID:9062895   (View Abstract at PubMed)

The alteration in calcium-binding protein regucalcin in the liver and serum of rats with streptozotocin (STZ)-diabetic state or ethanol ingestion was investigated. STZ (6.0 mg/100 g body weight) was subcutaneously administered in rats, and 1 or 3 weeks later they were sacrificed by bleeding. Liver regucalcin mRNA levels were not clearly altered by the diabetic state, as evidenced by Northern blotting using regucalcin cDNA (0.9 kb of open reading frame). Based on enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG, hepatic regucalcin concentration was decreased about 50% of control levels by STZ treatment. However, serum regucalcin concentration was not significantly altered by STZ treatment. Meanwhile, when rats ingested ethanol (10 and 30%) in the drinking water for 2 weeks, liver regucalcin mRNA levels were clearly increased, although hepatic regucalcin concentration was significantly decreased. Serum regucalcin concentration was not appreciably altered. Serum transaminases (GOT and GPT) activities were significantly increased at 1 or 3 weeks after STZ administration in rats, while their activities were not altered by ethanol ingestion. The present study demonstrates that hepatic regucalcin concentration is decreased independent of mRNA expression in the STZ-diabetes and during ethanol ingestion in rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Diabetes Mellitus  ISORgn (Rattus norvegicus)9590177; 9590177protein:decreased expression:liverRGD 
Experimental Diabetes Mellitus  IEP 9590177protein:decreased expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Rgn  (regucalcin)

Genes (Mus musculus)
Rgn  (regucalcin)

Genes (Homo sapiens)
RGN  (regucalcin)


Additional Information