RGD Reference Report - Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas. - Rat Genome Database

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Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.

Authors: Miura, S  Maesawa, C  Shibazaki, M  Yasuhira, S  Kasai, S  Tsunoda, K  Maeda, F  Takahashi, K  Akasaka, T  Masuda, T 
Citation: Miura S, etal., Am J Dermatopathol. 2014 Mar;36(3):211-6. doi: 10.1097/DAD.0b013e3182964e02.
RGD ID: 9590069
Pubmed: PMID:24658378   (View Abstract at PubMed)
DOI: DOI:10.1097/DAD.0b013e3182964e02   (Journal Full-text)

Methylation and demethylation of histone H3 lysine 9 (H3K9) play a role in the transcriptional regulation of several cancer-related genes and are closely associated with malignant tumor behavior. A novel study has recently demonstrated that SETDB1, a member of the H3K9 methyltransferases, accelerates tumor formation significantly in a zebrafish melanoma model. However, the expression of H3K9 methyltransferases including SETDB1 and demethylases has not been systematically examined in samples of human melanoma. Here, we used immunohistochemistry to examine the expression of the H3K9 methyltransferases, EHMT2 and SETDB1, and a H3K9 demethylase, LSD1, in 67 patients with melanoma. Overexpression of EHMT2, SETDB1, and LSD1 was observed in 14 (21%), 38 (57%), and 53 (79%) of the 67 patients, respectively. A significant relationship was observed between overexpression of EHMT2 or SETDB1 and aggressive tumor behavior such as lymph node metastasis and/or distant metastasis (P < 0.05), whereas no significant relationship was evident for LSD1 immunoreactivity. Univariate log-rank tests demonstrated that patients with melanoma overexpressing EHMT2 had a poorer outcome (P < 0.001), whereas overexpression of SETDB1 or LSD1 had no prognostic impact. These results suggest that overexpression of EHMT2 might be a prognostic marker in patients with melanoma.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Lymphatic Metastasis  IEP 9590069associated with MelanomaRGD 
Lymphatic Metastasis  ISOEHMT2 (Homo sapiens)9590069; 9590069associated with MelanomaRGD 
melanoma disease_progressionIEP 9590069 RGD 
melanoma disease_progressionISOEHMT2 (Homo sapiens)9590069; 9590069 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ehmt2  (euchromatic histone lysine methyltransferase 2)

Genes (Mus musculus)
Ehmt2  (euchromatic histone lysine N-methyltransferase 2)

Genes (Homo sapiens)
EHMT2  (euchromatic histone lysine methyltransferase 2)


Additional Information