RGD Reference Report - Transcriptional profiling of spinal cord injury-induced central neuropathic pain. - Rat Genome Database

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Transcriptional profiling of spinal cord injury-induced central neuropathic pain.

Authors: Nesic, O  Lee, J  Johnson, KM  Ye, Z  Xu, GY  Unabia, GC  Wood, TG  McAdoo, DJ  Westlund, KN  Hulsebosch, CE  Regino Perez-Polo, J 
Citation: Nesic O, etal., J Neurochem. 2005 Nov;95(4):998-1014. Epub 2005 Oct 10.
RGD ID: 8695957
Pubmed: PMID:16219025   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2005.03462.x   (Journal Full-text)

Central neuropathic pain (CNP) is an important problem following spinal cord injury (SCI), because it severely affects the quality of life of SCI patients. As in the patient population, the majority of rats develop significant allodynia (CNP rats) after moderate SCI. However, about 10% of SCI rats do not develop allodynia, or develop significantly less allodynia than CNP rats (non-CNP rats). To identify transcriptional changes underlying CNP development after SCI, we used Affymetrix DNA microarrays and RNAs extracted from the spinal cords of CNP and non-CNP rats. DNA microarry analysis showed significantly increased expression of a number of genes associated with inflammation and astrocytic activation in the spinal cords of rats that developed CNP. For example, mRNA levels of glial fibrilary acidic protein (GFAP) and Aquaporin 4 (AQP4) significantly increased in CNP rats. We also found that GFAP, S100beta and AQP4 protein elevation persisted for at least 9 months throughout contused spinal cords, consistent with the chronic nature of CNP. Thus, we hypothesize that CNP development results, in part, from dysfunctional, chronically "over-activated" astrocytes. Although, it has been shown that activated astrocytes are associated with peripheral neuropathic pain, this has not previously been demonstrated in CNP after SCI.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Hyperalgesia  ISOAqp4 (Rattus norvegicus)8695957; 8695957associated with Spinal Cord Injuries more ...RGD 
Hyperalgesia  IEP 8695957; 8695957associated with Spinal Cord Injuries more ...RGD 
Hyperalgesia  ISOGfap (Rattus norvegicus)8695957; 8695957associated with Spinal Cord Injuries more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aqp4  (aquaporin 4)
Gfap  (glial fibrillary acidic protein)

Genes (Mus musculus)
Aqp4  (aquaporin 4)
Gfap  (glial fibrillary acidic protein)

Genes (Homo sapiens)
AQP4  (aquaporin 4)
GFAP  (glial fibrillary acidic protein)


Additional Information