RGD Reference Report - Histone variants in rat spermatogonia and primary spermatocytes. - Rat Genome Database

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Histone variants in rat spermatogonia and primary spermatocytes.

Authors: Meistrich, ML  Bucci, LR  Trostle-Weige, PK  Brock, WA 
Citation: Meistrich ML, etal., Dev Biol. 1985 Nov;112(1):230-40.
RGD ID: 8693729
Pubmed: PMID:3932111   (View Abstract at PubMed)

The levels and synthesis of histone variants have been directly measured in spermatogonia and in various stages of primary spermatocytes purified from the rat testis. These measurements were made possible by the development of a procedure, employing centrifugal elutriation and density gradient centrifugation, to separate highly enriched populations of such cells from immature rat testes at the early stages of spermatogenesis. The results show a difference in regulation of the synthesis and accumulation of testis-specific histones H1t, TH2A, TH2B, and TH3. TH3 is present and actively synthesized in A and B spermatogonia. The testis-enriched variants, H2A.X and H1a, are also present at their maximal levels in A spermatogonia. No detectable amounts of H1t, and at most, low levels of TH2A and TH2B could be found in spermatogonia. While TH2A and TH2B are already present and actively synthesized in early primary spermatocytes (around the preleptotene stage), H1t does not accumulate until the pachytene stage.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
spermatogenesis  IEP 8693729 RGD 

Objects Annotated

Genes (Rattus norvegicus)
H2ax  (H2A.X variant histone)


Additional Information