RGD Reference Report - Catechol-O-methyltransferase (COMT) in rat brain: immunoelectron microscopic study with an antiserum against rat recombinant COMT protein. - Rat Genome Database

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Catechol-O-methyltransferase (COMT) in rat brain: immunoelectron microscopic study with an antiserum against rat recombinant COMT protein.

Authors: Karhunen, T  Tilgmann, C  Ulmanen, I  Panula, P 
Citation: Karhunen T, etal., Neurosci Lett. 1995 Feb 24;187(1):57-60.
RGD ID: 8662339
Pubmed: PMID:7617303   (View Abstract at PubMed)

Localization of catechol-O-methyltransferase (COMT) in rat cerebral cortex, neostriatum and cerebellar cortex was studied with preembedding immunoelectron microscopy using a specific antiserum raised against rat recombinant COMT protein. In all areas, immunoreactivity was found both in astrocytes and in neuronal processes. Reaction product was seen in the cytoplasm and in association with tubular structures of dendritic processes. Immunoreactivity was also located postsynaptically in dendritic spines and associated with the postsynaptic membrane. Strong immunoreaction was also seen in the cytoplasm of ependymal cells lining the ventricles, and in tanycytes in median eminence. The results suggest that postsynaptic dendritic spines and astrocytic processes may be the sites of catecholamine inactivation by COMT in rat brain.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendritic spine  IDA 8662339 RGD 
glutamatergic synapse is_active_inIDA 8662339PMID:7617303SynGO 
postsynapse is_active_inIDA 8662339PMID:7617303SynGO 
postsynaptic membrane  IDA 8662339 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Comt  (catechol-O-methyltransferase)


Additional Information